Posted by
Alison J. North on
URL: http://confocal-microscopy-list.275.s1.nabble.com/STEDYCON-user-experience-feedback-tp7588285p7588289.html
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Hi Stan,
Fortunately we had a very different experience to Christian last year
when they brought the STEDYCON to the OMIBS course at Woods Hole.
Karsten had it up and running and working perfectly within 1 hour of
unpacking the boxes, and we were collecting fabulous images that same
evening. I agree with the comments about having to use appropriate far
red and red dyes for the 775 nm laser - we used STAR Red and Alexa 594 -
and I also agree that the confocal images aren't as good as on a regular
confocal system, but that didn't worry me too much as I would never
intend to use it for that purpose. I can't comment on the
photoconversion question as I must admit I didn't look for that and we
were only using a few samples as we had limited time.
We also had the full blown STED system on demo in my lab for around 3
weeks in early 2017, and I found their service to be very good - they
were very helpful in trying to fix any problems.
I guess this is typical of demos - it always seems to be the case that
one goes well and another not so well...
All the best,
Alison
On 5/23/2018 8:40 AM, Christian Feldhaus wrote:
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> Hi,
>
> we had a Stedycon demo end of last year for 3 weeks. We could only
> test it for 1 week as abberior couldn't get it into working condition
> for 2 weeks. We have been very disappointed with the quality of their
> service.
>
> As Josef already mentioned the device is quite restricted in what it
> can do. Furthermore, we observed photoconversion of the STAR RED dye
> which can make interpreting your results quite an adventure.
>
> If you want more details about our test please feel free to contact me
> off list.
>
> Best,
>
> Christian
>
>
> On 22.05.2018 23:34, Stanislav Vitha wrote:
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>>
>> Howdy,
>>
>> we are considering the Abberior Instruments STEDYCON shoebox STED
>> system as an entry into superresolution for our core facility. We
>> have not had the opportunity to see it in action yet and would
>> appreciate your feedback/comments if you have used it or are using it
>> currently.
>>
>> Thank you in advance!
>>
>> Best Regards,
>>
>> Stan Vitha
>> Microscopy and Imaging Center
>> Texas A&M University
>
--
Alison J. North, Ph.D.,
Research Associate Professor and
Senior Director of the Bio-Imaging Resource Center,
The Rockefeller University,
1230 York Avenue,
New York,
NY 10065.
Tel: office ++ 212 327 7488
Tel: lab ++ 212 327 7486
Fax: ++ 212 327 7489