Re: Safe to run multiphoton laser at 100% power for overnight continuous imaging?

Posted by Craig Brideau on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Safe-to-run-multiphoton-laser-at-100-power-for-overnight-continuous-imaging-tp7588726p7588727.html

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

How are you not destroying your sample at these powers and timescales? This
seems rather risky to me.

Craig

On Tue, Sep 25, 2018, 6:56 AM Moulding, Dale <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear list,
>
> I've been asked to run our 2 photon laser (Mai Tai eHP DeepSee) at full
> power (3W) non-stop over night to scan a large sample.
> When doing ablations we run at 100% and I hadn't even given it a second
> thought, as the ablations are over in a flash.
> However, continuously (4 to 16 hours) running 100% 800nM at 3W I'm
> concerned we may damage the optics of the system. It will be on a Zeiss
> Examiner, going through Zeiss's 10x0.5 or 20x1.0 water dippers.
> Has anyone ever imaged / ablated with a 2P laser continuously at full
> power?
> Should I be concerned that the objectives may be damaged?
>
> Cheers
>
> Dale
>
>
> Dale Moulding PhD FRMS
> ICH Light Microscopy Facility
> Room W2.06, BDRC office
> UCL Great Ormond Street Institute of Child Health
> 30 Guilford St
> London WC1N 1EH
> Mob: [phone]
> Tel: [phone]
>
> http://www.ucl.ac.uk/ich/core-scientific-facilities-centres/confocal-microscopy
>