Re: Safe to run multiphoton laser at 100% power for overnight continuous imaging?

Posted by Armstrong, Brian on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Safe-to-run-multiphoton-laser-at-100-power-for-overnight-continuous-imaging-tp7588726p7588729.html

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Hi Dale, it depends in part on the wavelength. As I imagine you already know the output power at say 1050nm is far lower than say 800nm. I do not think the LASER "cares" what your output power is. As for the optics I cannot say. I think that they fall off in transmittance above a given wavelength in the IR range but I do not know if this is because of a coating or properties of the lens??? I have heard stories of people burning objectives. Pockels cells will also burn out after around 10 years or so.

P.S. How do you like the 10x/0.5 W for 2P work?

Brian Armstrong PhD
Associate Research Professor
Developmental and Stem Cell Biology
Diabetes and Metabolic Diseases
Director, Light Microscopy Core
Beckman Research Institute, City of Hope



-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Moulding, Dale
Sent: Tuesday, September 25, 2018 5:56 AM
To: [hidden email]
Subject: Safe to run multiphoton laser at 100% power for overnight continuous imaging?

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Dear list,

I've been asked to run our 2 photon laser (Mai Tai eHP DeepSee) at full power (3W) non-stop over night to scan a large sample.
When doing ablations we run at 100% and I hadn't even given it a second thought, as the ablations are over in a flash.
However, continuously (4 to 16 hours) running 100% 800nM at 3W I'm concerned we may damage the optics of the system. It will be on a Zeiss Examiner, going through Zeiss's 10x0.5 or 20x1.0 water dippers.
Has anyone ever imaged / ablated with a 2P laser continuously at full power?
Should I be concerned that the objectives may be damaged?

Cheers

Dale


Dale Moulding PhD FRMS
ICH Light Microscopy Facility
Room W2.06, BDRC office
UCL Great Ormond Street Institute of Child Health
30 Guilford St
London WC1N 1EH
Mob: [phone]
Tel: [phone]
http://www.ucl.ac.uk/ich/core-scientific-facilities-centres/confocal-microscopy

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