Posted by
Claire Mitchell on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Safe-to-run-multiphoton-laser-at-100-power-for-overnight-continuous-imaging-tp7588726p7588730.html
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Hi Dale
We suffered a cracked 10x air objective on our Zeiss 7MP with ~4W Chameleon and this was without imaging at max power for significant amounts of time, only occasional 100% ablation. Luckily Zeiss agreed with me that the objective should be able to withstand the full power of the supplied laser and replaced it but maybe not a good idea if you don't have a service contract?
Best wishes
Claire
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Subject: Re: Safe to run multiphoton laser at 100% power for overnight continuous imaging?
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Hello Dale,
I've ran a 2-P Mai Tai in the past for over 16 hours continuously at 1W power for a long imaging session. I did have a pockel cell to control the power that was actually getting to the sample but in terms of the laser being able to run for that long I had no issues. I haven't notice any damage to the optics so far either. The only problem I've ran into was a loss of communication between the laser and the computer with the control software. Hope that helps.
Lorenzo
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Dear list,
I've been asked to run our 2 photon laser (Mai Tai eHP DeepSee) at full power (3W) non-stop over night to scan a large sample.
When doing ablations we run at 100% and I hadn't even given it a second thought, as the ablations are over in a flash.
However, continuously (4 to 16 hours) running 100% 800nM at 3W I'm concerned we may damage the optics of the system. It will be on a Zeiss Examiner, going through Zeiss's 10x0.5 or 20x1.0 water dippers.
Has anyone ever imaged / ablated with a 2P laser continuously at full power?
Should I be concerned that the objectives may be damaged?
Cheers
Dale
Dale Moulding PhD FRMS
ICH Light Microscopy Facility
Room W2.06, BDRC office
UCL Great Ormond Street Institute of Child Health
30 Guilford St
London WC1N 1EH
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