Re: Safe to run multiphoton laser at 100% power for overnight continuous imaging?

Posted by Craig Brideau on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Safe-to-run-multiphoton-laser-at-100-power-for-overnight-continuous-imaging-tp7588726p7588731.html

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

You do raise a valid point Brian, the power of most Ti:Saphs varies fairly
dramatically by wavelength. The transmission through the optical system
will also be different, and what the system considers '100%' may not
actually be that much power under certain situations. In most cases,
however, a Ti:Saph can produce much more power than is necessary for
imaging.
Try measuring the power out of the objective when set to 100% to see what
you are actually getting; maybe it isn't that much compared to the output
of the laser once all the system losses are considered.

Craig

On Tue, Sep 25, 2018 at 9:41 AM Armstrong, Brian <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi Dale, it depends in part on the wavelength. As I imagine you already
> know the output power at say 1050nm is far lower than say 800nm. I do not
> think the LASER "cares" what your output power is. As for the optics I
> cannot say. I think that they fall off in transmittance above a given
> wavelength in the IR range but I do not know if this is because of a
> coating or properties of the lens??? I have heard stories of people burning
> objectives. Pockels cells will also burn out after around 10 years or so.
>
> P.S. How do you like the 10x/0.5 W for 2P work?
>
> Brian Armstrong PhD
> Associate Research Professor
> Developmental and Stem Cell Biology
> Diabetes and Metabolic Diseases
> Director, Light Microscopy Core
> Beckman Research Institute, City of Hope
>
>
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[hidden email]]
> On Behalf Of Moulding, Dale
> Sent: Tuesday, September 25, 2018 5:56 AM
> To: [hidden email]
> Subject: Safe to run multiphoton laser at 100% power for overnight
> continuous imaging?
>
> [Attention: This email came from an external source. Do not open
> attachments or click on links from unknown senders or unexpected emails.]
>
>
>
>
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear list,
>
> I've been asked to run our 2 photon laser (Mai Tai eHP DeepSee) at full
> power (3W) non-stop over night to scan a large sample.
> When doing ablations we run at 100% and I hadn't even given it a second
> thought, as the ablations are over in a flash.
> However, continuously (4 to 16 hours) running 100% 800nM at 3W I'm
> concerned we may damage the optics of the system. It will be on a Zeiss
> Examiner, going through Zeiss's 10x0.5 or 20x1.0 water dippers.
> Has anyone ever imaged / ablated with a 2P laser continuously at full
> power?
> Should I be concerned that the objectives may be damaged?
>
> Cheers
>
> Dale
>
>
> Dale Moulding PhD FRMS
> ICH Light Microscopy Facility
> Room W2.06, BDRC office
> UCL Great Ormond Street Institute of Child Health
> 30 Guilford St
> London WC1N 1EH
> Mob: [phone]
> Tel: [phone]
>
> http://www.ucl.ac.uk/ich/core-scientific-facilities-centres/confocal-microscopy
>
> ------------------------------------------------------------
> -SECURITY/CONFIDENTIALITY WARNING-
>
> This message and any attachments are intended solely for the individual or
> entity to which they are addressed. This communication may contain
> information that is privileged, confidential, or exempt from disclosure
> under applicable law (e.g., personal health information, research data,
> financial information). Because this e-mail has been sent without
> encryption, individuals other than the intended recipient may be able to
> view the information, forward it to others or tamper with the information
> without the knowledge or consent of the sender. If you are not the intended
> recipient, or the employee or person responsible for delivering the message
> to the intended recipient, any dissemination, distribution or copying of
> the communication is strictly prohibited. If you received the communication
> in error, please notify the sender immediately by replying to this message
> and deleting the message and any accompanying files from your system. If,
> due to the security risks, you do not wish to receive further
> communications via e-mail, please reply to this message and inform the
> sender that you do not wish to receive further e-mail from the sender.
> (LCP301)
> ------------------------------------------------------------
>