http://confocal-microscopy-list.275.s1.nabble.com/Safe-to-run-multiphoton-laser-at-100-power-for-overnight-continuous-imaging-tp7588726p7588732.html
Thanks for the replies. It will be at 800nM, so full power output, and will be continuously firing though the objective for at least 4 hours as it is not for imaging but for catalysis.
Zeiss aren't able to guarantee their objectives for this (drilling to the centre of the earth!), so if I do get a cracked lens / glue then I'm not sure our service contract will cover it.
@Lorenzo, for your 16 hour experiment were you sending the laser through the objective non-stop for the entire time, or just imaging periodically?
@Brian, I love the 10x0.5 dipper for 2P work. Massive improvement over the 10x0.3.
@Claire, I'm not sure Zeiss will cover the damage this time, and haven't really had a definitive answer, unless I've misunderstood them.
Subject: Re: Safe to run multiphoton laser at 100% power for overnight continuous imaging?
You do raise a valid point Brian, the power of most Ti:Saphs varies fairly dramatically by wavelength. The transmission through the optical system will also be different, and what the system considers '100%' may not actually be that much power under certain situations. In most cases, however, a Ti:Saph can produce much more power than is necessary for imaging.
Try measuring the power out of the objective when set to 100% to see what you are actually getting; maybe it isn't that much compared to the output of the laser once all the system losses are considered.
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> Hi Dale, it depends in part on the wavelength. As I imagine you
> already know the output power at say 1050nm is far lower than say
> 800nm. I do not think the LASER "cares" what your output power is. As
> for the optics I cannot say. I think that they fall off in
> transmittance above a given wavelength in the IR range but I do not
> know if this is because of a coating or properties of the lens??? I
> have heard stories of people burning objectives. Pockels cells will also burn out after around 10 years or so.
>
> P.S. How do you like the 10x/0.5 W for 2P work?
>
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> continuous imaging?
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> Dear list,
>
> I've been asked to run our 2 photon laser (Mai Tai eHP DeepSee) at
> full power (3W) non-stop over night to scan a large sample.
> When doing ablations we run at 100% and I hadn't even given it a
> second thought, as the ablations are over in a flash.
> However, continuously (4 to 16 hours) running 100% 800nM at 3W I'm
> concerned we may damage the optics of the system. It will be on a
> Zeiss Examiner, going through Zeiss's 10x0.5 or 20x1.0 water dippers.
> Has anyone ever imaged / ablated with a 2P laser continuously at full
> power?
> Should I be concerned that the objectives may be damaged?
>
> Cheers
>
> Dale
>
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> Dale Moulding PhD FRMS
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