Posted by
Armstrong, Brian on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Safe-to-run-multiphoton-laser-at-100-power-for-overnight-continuous-imaging-tp7588726p7588735.html
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Dale, thank you for the answers. Can you please update this post when you complete the experiment? Especially if you damage the lens.
Thanks in advance,
Brian Armstrong PhD
Associate Research Professor
Developmental and Stem Cell Biology
Diabetes and Metabolic Diseases
Director, Light Microscopy Core
Beckman Research Institute, City of Hope
-----Original Message-----
From: Confocal Microscopy List [mailto:
[hidden email]] On Behalf Of Moulding, Dale
Sent: Wednesday, September 26, 2018 2:00 AM
To:
[hidden email]
Subject: Re: Safe to run multiphoton laser at 100% power for overnight continuous imaging?
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Dear all,
Thanks for the replies. It will be at 800nM, so full power output, and will be continuously firing though the objective for at least 4 hours as it is not for imaging but for catalysis.
Zeiss aren't able to guarantee their objectives for this (drilling to the centre of the earth!), so if I do get a cracked lens / glue then I'm not sure our service contract will cover it.
@Lorenzo, for your 16 hour experiment were you sending the laser through the objective non-stop for the entire time, or just imaging periodically?
@Craig, agreed, it’s a lot of power, but not for imaging, for catalysis.
@Brian, I love the 10x0.5 dipper for 2P work. Massive improvement over the 10x0.3.
@Claire, I'm not sure Zeiss will cover the damage this time, and haven't really had a definitive answer, unless I've misunderstood them.
Cheers
Dale
-----Original Message-----
From: Confocal Microscopy List <
[hidden email]> On Behalf Of Craig Brideau
Sent: 25 September 2018 17:13
To:
[hidden email]
Subject: Re: Safe to run multiphoton laser at 100% power for overnight continuous imaging?
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You do raise a valid point Brian, the power of most Ti:Saphs varies fairly dramatically by wavelength. The transmission through the optical system will also be different, and what the system considers '100%' may not actually be that much power under certain situations. In most cases, however, a Ti:Saph can produce much more power than is necessary for imaging.
Try measuring the power out of the objective when set to 100% to see what you are actually getting; maybe it isn't that much compared to the output of the laser once all the system losses are considered.
Craig
On Tue, Sep 25, 2018 at 9:41 AM Armstrong, Brian <
[hidden email]> wrote:
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>
> Hi Dale, it depends in part on the wavelength. As I imagine you
> already know the output power at say 1050nm is far lower than say
> 800nm. I do not think the LASER "cares" what your output power is. As
> for the optics I cannot say. I think that they fall off in
> transmittance above a given wavelength in the IR range but I do not
> know if this is because of a coating or properties of the lens??? I
> have heard stories of people burning objectives. Pockels cells will also burn out after around 10 years or so.
>
> P.S. How do you like the 10x/0.5 W for 2P work?
>
> Brian Armstrong PhD
> Associate Research Professor
> Developmental and Stem Cell Biology
> Diabetes and Metabolic Diseases
> Director, Light Microscopy Core
> Beckman Research Institute, City of Hope
>
>
>
> -----Original Message-----
> From: Confocal Microscopy List
> [mailto:
[hidden email]]
> On Behalf Of Moulding, Dale
> Sent: Tuesday, September 25, 2018 5:56 AM
> To:
[hidden email]
> Subject: Safe to run multiphoton laser at 100% power for overnight
> continuous imaging?
>
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>
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy> Post images on
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>
> Dear list,
>
> I've been asked to run our 2 photon laser (Mai Tai eHP DeepSee) at
> full power (3W) non-stop over night to scan a large sample.
> When doing ablations we run at 100% and I hadn't even given it a
> second thought, as the ablations are over in a flash.
> However, continuously (4 to 16 hours) running 100% 800nM at 3W I'm
> concerned we may damage the optics of the system. It will be on a
> Zeiss Examiner, going through Zeiss's 10x0.5 or 20x1.0 water dippers.
> Has anyone ever imaged / ablated with a 2P laser continuously at full
> power?
> Should I be concerned that the objectives may be damaged?
>
> Cheers
>
> Dale
>
>
> Dale Moulding PhD FRMS
> ICH Light Microscopy Facility
> Room W2.06, BDRC office
> UCL Great Ormond Street Institute of Child Health
> 30 Guilford St
> London WC1N 1EH
> Mob: 07787 699609
> Tel: 020 7905 2753
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http://www.ucl.ac.uk/ich/core-scientific-facilities-centres/confocal-m> icroscopy
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