this paper on 280 fluorophores tested by MPEF by PubMed related article (sort by date) or related article (sort by date) ... Re: How to keep up with the latest literature?

Posted by George McNamara on
URL: http://confocal-microscopy-list.275.s1.nabble.com/How-to-keep-up-with-the-latest-literature-tp7588767p7588780.html

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Re: How to keep up with the latest literature?

this paper on 280 fluorophores tested by MPEF by PubMed related article
(sort by date) or related article (sort by date) ... of a search
involving fast photon counting fluorescence confocal microscopy ...
after submitted CZI imaging scientist proposal to focus attention on
using 'FPC' to get faster, more quantitative data -- my thanks to Leong
for posting the RFA here and organizing and hosting the microscopy
core's meeting that encouraged CZI to initiate the program.


Ricard C(1)(2)(3), Arroyo ED(4), He CX(4), Portera-Cailliau C(4)(5), Lepousez G(6), Canepari M(7)(8)(9), Fiole D(10)(11)(12).

Two-photon probes for in vivo multicolor microscopy of the structure and signals of brain cells.

Brain Struct Funct. 2018 Sep;223(7):3011-3043. doi: 10.1007/s00429-018-1678-1. Epub 2018 May 11.

Imaging the brain of living laboratory animals at a microscopic scale
can be achieved by two-photon microscopy thanks to the high
penetrability and low phototoxicity of the excitation wavelengths used.
However, knowledge of the two-photon spectral properties of the myriad
fluorescent probes is generally scarce and, for many, non-existent. In
addition, the use of different measurement units in published reports
further hinders the design of a comprehensive imaging experiment. In
this review, we compile and homogenize the two-photon spectral
properties of 280 fluorescent probes. We provide practical data,
including the wavelengths for optimal two-photon excitation, the peak
values of two-photon action cross section or molecular brightness, and
the emission ranges. Beyond the spectroscopic description of these
fluorophores, we discuss their binding to biological targets. This
specificity allows in vivo imaging of cells, their processes, and even
organelles and other subcellular structures in the brain. In addition to
probes that monitor endogenous cell metabolism, studies of healthy and
diseased brain benefit from the specific binding of certain probes to
pathology-specific features, ranging from amyloid-β plaques to the
autofluorescence of certain antibiotics. A special focus is placed on
functional in vivo imaging using two-photon probes that sense specific
ions or membrane potential, and that may be combined with optogenetic
actuators. Being closely linked to their use, we examine the different
routes of intravital delivery of these fluorescent probes according to
the target. Finally, we discuss different approaches, strategies, and
prerequisites for two-photon multicolor experiments in the brains of
living laboratory animals.
DOI: 10.1007/s00429-018-1678-1
PMCID: PMC6119111 [Available on 2019-09-01]
PMID: 29748872

supplemental file:

Biophysical properties of two-photon–suitable probes in alphabetical
order: peak wavelength of two-photon action cross section (/λ/_2PA );
peak two-photon action cross section (/σ/_2 /φ/) ; peak wavelength of
molecular brightness (/λ/_ε_max ) ; peak molecular brightness (/ε/_max )
; fluorescence wavelength (/λ/_fluo ). (1) : calculated from the/σ/_2
and/φ/values (2) : data from commercial provider (XLSX 45 KB)




On 10/2/2018 2:53 AM, Kai Schleicher wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear confocal list,
>
> How do you keep up with the latest publications on microscopy,
> imaging, new fluoresecent proteins, new techniques etc?
>
> Are there maybe a few blogs you would suggest?
>
> This topic is cross-posted on twitter here:
> https://twitter.com/imcf_basel/status/1046703274886397952
>
> Cheers, Kai
>