Posted by
Craig Brideau on
URL: http://confocal-microscopy-list.275.s1.nabble.com/thermal-imaging-through-microscope-tp7588813p7588815.html
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BK7 optical glass, which is pretty common in microscope optics, drops off
fairly sharply in transmission by 2 to 2.5um, so that will be a problem.
The last time I heard about a lab attempting thermal imaging they had a
custom gold-coated reflective objective constructed for the purpose, and
designed all their other optics with IR glass like zinc selenide or
similar. It seems unlikely this would work at all through a conventional
microscope. I can point you to some additional resources if you are
interested.
Craig
On Fri, Oct 12, 2018 at 12:17 PM Kathryn Spencer <
[hidden email]>
wrote:
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http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy> Post images on
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> Hi;
> I'm looking for suggestions and equipment to do thermal
> imaging through a microscope. We want to image C. elegans to look at their
> body temperature under different metabolic conditions. While I know they
> are poikilotherms, we believe they will show a difference to their local
> background under these conditions. Can you recommend a decent camera for
> thermal imaging? Are there IR cutoff filters or optics that will not
> transmit IR wavelengths in a basic fluorescence-type microscope (needed for
> worm-sized resolution ~10x mag)?
> Thanks in advance.
> Kathy Spencer
>
> The Scripps Research Institute
> Dept of Molecular and Cellular Neuroscience
> 10550 N. Torrey Pines Road
> DNC 216
> La Jolla, Ca 92037
>