Re: thermal imaging through microscope

Posted by Mark Cannell-2 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/thermal-imaging-through-microscope-tp7588813p7588816.html

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I suggest you do a calculation as to what temperature difference you expect -use the metabolic rate of say reptiles and solve the heat equation for a cylinder of the appropriate size. I suspect the delta t is very very small.

HTH

Mark B. Cannell. Ph.D. FRSNZ FISHR
Department of Physiology, Pharmacology & Neuroscience
School of Medical Sciences
University Walk
Bristol BS8 1TD
 
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On 12/10/18, 7:17 PM, "Confocal Microscopy List on behalf of Kathryn Spencer" <[hidden email] on behalf of [hidden email]> wrote:

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    Hi;
                    I'm looking for suggestions and equipment to do thermal imaging through a microscope. We want to image C. elegans to look at their body temperature under different metabolic conditions. While I know they are poikilotherms, we believe they will show a difference to their local background under these conditions. Can you recommend a decent camera for thermal imaging? Are there IR cutoff filters or optics that will not transmit IR wavelengths in a basic fluorescence-type microscope (needed for worm-sized resolution ~10x mag)?
                    Thanks in advance.
                    Kathy Spencer
   
    The Scripps Research Institute
    Dept of Molecular and Cellular Neuroscience
    10550 N. Torrey Pines Road
    DNC 216
    La Jolla, Ca 92037