http://confocal-microscopy-list.275.s1.nabble.com/thermal-imaging-through-microscope-tp7588813p7588821.html
You could always ask for a demo and see if it works. They have an
> *****
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>
> I have seen Stage to CO2 incubator companies publish data of temperature
> gradients on the 96 well plate precisely
> Ganesh
>
> Sent from my iPhone
>
> > On 13-Oct-2018, at 10:08 AM, Karel Zuzak <
[hidden email]> wrote:
> >
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> >
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> posting.
> > *****
> >
> > Dear Kathy,
> >
> > I am not aware of any commercially available thermal microscope but that
> doesn’t mean it doesn’t exist.
> >
> > While at NIH In the late 90s our lab was exploring various military
> technologies for medical application and thermal imaging was one.
> >
> > As you might imagine there have been great advances in thermal imaging
> as well as NIR based night vision. The advantage of thermal is that it is
> passive. Meaning one does not need a source. Your subject is the source.
> Unlike fluorescence or NIR where one must expose the subject to an
> excitation wavelength or NIR illumination.
> >
> > So thermal is great but we found a lot of subject to subject variability
> in temperature which may have been from the ambient surrounding or one of
> the many physiologic factors influencing temperature.
> >
> > The other difficulty with thermal was the need for expensive custom
> optics.
> >
> > So please know once you get a thermal system working you may encounter a
> lot of variability from your experiments that will require a lot of control
> to separate the potentially different sources to metabolism.
> >
> > Regarding the optics it’s a question of your budget. Back in the 1990s a
> system could easily cost over 100k for the camera alone and needed constant
> liquid nitrogen. The reason being thermal emission occurs around 7 to 14
> um. Assuming I’m remembering correctly. A standard ccd or cmos detector
> won’t detect these wavelengths and standard glass optics won’t transmit
> those wavelengths sufficiently. So one must use specialized focal plane
> arrays and optics along with imaging methods using long integration or
> exposure times or co-adds etc. Then if you need greater magnification and
> need microscope optics then you need to be sure all the optics in that
> microscope light path will transmit the thermal wavelengths.
> >
> > That said great advances have been made in the thermal field. One can
> pick up a point/shoot IR thermometer at the hardware store. As an example
> the cost of consumer thermal imaging scopes for hunting applications are
> down to 5k where previously they could cost up around 80k. I have seen
> some as low as 700 but the image resolution and thermal variability
> reflect that price.
> >
> > So if you are set on doing this I would suggest knowing your continuum;
> the thermal/temperature and imaging sensitivity and resolution. Then I
> would start with a call to FLIR. They make a variety of thermal and NIR
> cameras and can possibly help you out. Another place you might like to call
> are microscope companies such as Zeiss, Nikon, Olympus etc and see what
> they have to offer.
> >
> > If after all this you are still interested and have the budget. I’m
> happy to work with you toward finding/developing a custom solution.
> >
> > Warm Regards
> > Karel Zuzak
> >
[hidden email]
> >
[hidden email]
> >
> > 🤠
> >
> >
> > On Oct 12, 2018, at 3:05 PM, Craig Brideau <
[hidden email]>
> wrote:
> >
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> >
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy> > Post images on
http://www.imgur.com and include the link in your
> posting.
> > *****
> >
> > BK7 optical glass, which is pretty common in microscope optics, drops off
> > fairly sharply in transmission by 2 to 2.5um, so that will be a problem.
> > The last time I heard about a lab attempting thermal imaging they had a
> > custom gold-coated reflective objective constructed for the purpose, and
> > designed all their other optics with IR glass like zinc selenide or
> > similar. It seems unlikely this would work at all through a conventional
> > microscope. I can point you to some additional resources if you are
> > interested.
> >
> > Craig
> >
> > On Fri, Oct 12, 2018 at 12:17 PM Kathryn Spencer <
[hidden email]>
> > wrote:
> >
> >> *****
> >> To join, leave or search the confocal microscopy listserv, go to:
> >>
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy> >> Post images on
http://www.imgur.com and include the link in your
> posting.
> >> *****
> >>
> >> Hi;
> >> I'm looking for suggestions and equipment to do thermal
> >> imaging through a microscope. We want to image C. elegans to look at
> their
> >> body temperature under different metabolic conditions. While I know they
> >> are poikilotherms, we believe they will show a difference to their local
> >> background under these conditions. Can you recommend a decent camera for
> >> thermal imaging? Are there IR cutoff filters or optics that will not
> >> transmit IR wavelengths in a basic fluorescence-type microscope (needed
> for
> >> worm-sized resolution ~10x mag)?
> >> Thanks in advance.
> >> Kathy Spencer
> >>
> >> The Scripps Research Institute
> >> Dept of Molecular and Cellular Neuroscience
> >> 10550 N. Torrey Pines Road
> >> DNC 216
> >> La Jolla, Ca 92037
> >>
>
Benjamin E. Smith, Ph. D.