http://confocal-microscopy-list.275.s1.nabble.com/thermal-imaging-through-microscope-tp7588813p7588824.html
Another exotic idea, comparing stokes and anti-stokes Raman intensities and derive temperature from this? I heard that this is used for combustion engines, but not sure how sensitive and if the fish will survive the high laser powers.
> On 13 Oct 2018, at 13:50, Masur, Sandra <
[hidden email]> wrote:
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> Direct thermal imaging references:
>
>
> 1. Itoh H, Arai S, Sudhaharan T, Lee S-C, Chang Y-T, Ishiwata Si, et al. Direct organelle thermometry with fluorescence lifetime imaging microscopy in single myotubes. Chemical Communications. 2016;52(24):4458-61.
>
> 2. Arai S, Suzuki M, Park S-J, Yoo JS, Wang L, Kang N-Y, et al. Mitochondria-targeted fluorescent thermometer monitors intracellular temperature gradient. Chemical Communications. 2015;51(38):8044-7.
>
> 3. Arai S, Lee S-C, Zhai D, Suzuki M, and Chang YT. A molecular fluorescent probe for targeted visualization of temperature at the endoplasmic reticulum. Scientific reports. 2014;4:6701.
>
> 4. Hayashi T, Fukuda N, Uchiyama S, and Inada N. A cell-permeable fluorescent polymeric thermometer for intracellular temperature mapping in mammalian cell lines. PloS one. 2015;10(2):e0117677.
>
> 5. Kiyonaka S, Kajimoto T, Sakaguchi R, Shinmi D, Omatsu-Kanbe M, Matsuura H, et al. Genetically encoded fluorescent thermosensors visualize subcellular thermoregulation in living cells. Nature methods. 2013;10(12):1232-8.
>
> HTH
>
> Sandy
> __________________________
> Sandra K. Masur, PhD
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> On Oct 12, 2018, at 4:10 PM, Mark Cannell <
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> I suggest you do a calculation as to what temperature difference you expect -use the metabolic rate of say reptiles and solve the heat equation for a cylinder of the appropriate size. I suspect the delta t is very very small.
>
> HTH
>
> Mark B. Cannell. Ph.D. FRSNZ FISHR
> Department of Physiology, Pharmacology & Neuroscience
> School of Medical Sciences
> University Walk
> Bristol BS8 1TD
>
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[hidden email]
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> On 12/10/18, 7:17 PM, "Confocal Microscopy List on behalf of Kathryn Spencer" <
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> Hi;
> I'm looking for suggestions and equipment to do thermal imaging through a microscope. We want to image C. elegans to look at their body temperature under different metabolic conditions. While I know they are poikilotherms, we believe they will show a difference to their local background under these conditions. Can you recommend a decent camera for thermal imaging? Are there IR cutoff filters or optics that will not transmit IR wavelengths in a basic fluorescence-type microscope (needed for worm-sized resolution ~10x mag)?
> Thanks in advance.
> Kathy Spencer
>
> The Scripps Research Institute
> Dept of Molecular and Cellular Neuroscience
> 10550 N. Torrey Pines Road
> DNC 216
> La Jolla, Ca 92037
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>