Re: FPs for MP

Posted by Gabriel KRENS-2 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/FPs-for-MP-tp7588839p7588841.html

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To add: Where you can also combine TagBFP + eGFP/Venus + Tetra-methyl Rhodamine (TMR), in case you are able to add labelled dextrans for instance, to excite 3 dyes with 830-840nm

Best
Gabriel


-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Ekaterina PAPUSHEVA
Sent: Tuesday, October 16, 2018 15:43
To: [hidden email]
Subject: Re: FPs for MP

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BFP/GFP and BFP/YFP worked well with 800-820 nm excitation in Zebrafish.

Good luck!
Ekaterina

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Graham Wright
Sent: Tuesday, October 16, 2018 3:38 PM
To: [hidden email]
Subject: FPs for MP

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Dear all,

I'm keen to find out what your favoured pair of fluorescent proteins for multiphoton microcopy, in which you’re limited to a single excitation wavelength (to avoid compromising too much with each). And which excitation wavelengths you use with them.

Thanks in advance,
Graham

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Graham Wright, PhD
Director, A*STAR Microscopy Platform

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