http://confocal-microscopy-list.275.s1.nabble.com/Vale-Jim-Pawley-tp7589279p7589298.html
Not to mention the famous 39 steps for quantification. I kept a hardcopy for years.
I was there in the 2001 edition with Stefan Hell giving a talk about STED. It was also the debut of the EMCCD cameras, a Roper. At least the first time I saw one, with Pawley making an improvised demo of the potentiality and explaining how it works.
> On 12 Mar 2019, at 18:31, Steffen Dietzel <
[hidden email]> wrote:
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
>
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy> Post images on
http://www.imgur.com and include the link in your posting.
> *****
>
> Yes, Renato, it was the very first of these courses that we both attended as students.
>
> I still have the green folder from the course in my office and just took it out to check: 3D Microscopy of Living Cells, July 27 - August 4, 1996. I learned a lot at this course, including that PMTs have less noise but worse QE than CCD cameras (from my notes on a talk by Jim), that slit scan confocals are possible (I believe Noran was there) and that you can put salmon on a barbecue. The course motto seems to have adapted later on, back then it was "If it's not diffraction its statistics" and I proudly wore the respective course T-shirt for quite some time.
>
> I also remember that upon returning from the course quite a few people were interested in the hand-out of Roger Tsiens talk that Jim was able to organize, with the spectrum of a new and yet unpublished GFP variant: "10C has the longest wavelengths yet (513 nm exc, 527 nm em)".
>
> I cannot even estimate the number of times I looked up something in the handbook.
>
> A lot of memories, all of them good ones. Thank you Jim! My thoughts are with the family.
>
> Steffen
>
>
> Am 12.03.2019 um 15:55 schrieb Renato Mortara:
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>>
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy>> Post images on
http://www.imgur.com and include the link in your posting.
>> *****
>>
>> I attended the 1996 course (the very first one?) and later on Jim came to Brazil for a confocal congress and we had a great time showing him around historic cities around Belo Horizonte.
>>
>> Will be greatly missed.
>>
>> Renato Mortara
>>
>>
>> -----Mensagem original-----
>> De: Confocal Microscopy List <
[hidden email]> Em nome de Cromey, Douglas W - (dcromey)
>> Enviada em: terça-feira, 12 de março de 2019 11:35
>> Para:
[hidden email]
>> Assunto: Re: Vale Jim Pawley
>>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>>
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy>> Post images on
http://www.imgur.com and include the link in your posting.
>> *****
>>
>> I attended the UBC course in 2008. My thanks to Jim and the many others who contributed to the course I attended (and all the other years that the course was held). The UBC course was an intellectually stimulating (and exhausting) 12 days. The Handbook is an essential reference and a lot of work on everyone's part. My heart goes out Jim's family.
>>
>> Thanks for letting the rest of the community know Steve.
>> Doug
>>
>> ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
>> Douglas W. Cromey, M.S. - Associate Scientific Investigator Dept. of Cellular & Molecular Medicine, University of Arizona
>> 1501 N. Campbell Ave, Tucson, AZ 85724-5044 USA
>>
>> office: LSN 463 email:
[hidden email]
>> voice: 520-626-2824 fax: 520-626-2097
>>
>>
http://swehsc.pharmacy.arizona.edu/micro>> Home of: "Microscopy and Imaging Resources on the WWW"
>>
>> UA Microscopy Alliance -
http://microscopy.arizona.edu/>>
>> -----Original Message-----
>> From: Confocal Microscopy List <
[hidden email]> On Behalf Of Stephen Cody
>> Sent: Tuesday, March 12, 2019 12:30 AM
>> To:
[hidden email]
>> Subject: Vale Jim Pawley
>>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>>
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy>> Post images on
http://www.imgur.com and include the link in your posting.
>> *****
>>
>> Very sad news. I just received this from Jim’s Facebook account and also from Christine Pawley.
>>
>> “We are heartbroken to announce the sudden loss of James Binfield Pawley, who collapsed playing tennis on Thursday March 7th and died almost immediately.
>> Jim was born January 15th 1944 in Gerrard’s Cross, England. He immigrated to Canada with his parents in 1946, first to Cloverdale, BC, where they joined his aunt Winifred and cousin Brenda on their chicken farm, and then to Vancouver. He spent summers on Gambier Island with the family of Jack and Joan Warn. In the late 1950s, the family moved to Ben Lomond, California. From 1962-66 he studied electrical engineering at the Carnegie Institute of Technology in Pittsburgh, and in 1972 he got his Ph.D. in biophysics at the University of California-Berkeley. After a series of postdoctoral positions (including in London, where he met his wife Christine), in 1978 he took a faculty position in the department of Zoology at the University of Wisconsin-Madison. One of his main responsibilities was to run the three-story million-volt electron microscope, part of a national microscopy facility.
>> For sixteen years he also directed the 3D Microscopy of Living Cells Course on the University of British Columbia’s campus. With a faculty of internationally known scientists and cutting edge equipment loaned by manufacturers, the 3D Microscopy of Living Cells (motto, “It’s not just diffraction, it’s not just statistics: It’s biology!”) attracted participants from all over the world. The course provided the foundation for his best-known publication, the Handbook of Biological Confocal Microscopy, now in its third edition and still an essential resource.
>> Galvanized by the threat of climate change, Jim spent recent years raising the alarm. He organized a teach-in at UW-Madison, and taught classes on climate change there and at the Elder College in Sechelt. He helped organize a climate march in Vancouver, marched in Washington DC, and gave lectures in many places including the Sunshine Coast, and Harbin, China. He was especially active in the Clean Air Society and the Sunshine Coast Community Solar Association; his letters often appeared in local papers.
>> Jim loved photography and music (especially Scarlatti); he loved to fix houses, furniture, and boats. In the late 1970s with friends, Jim built a cabin across the water from Egmont (boat access only). The family spent almost every summer there, trekking 2000 miles each way by car. He was a founder of the Doriston Music Festival, which in its first year was his kids on violins and keyboard, and Don and George Gilmour on mandolin and guitar. When Christine retired in 2012, the couple moved to the house they built in Sechelt. Jim loved the coast, and was so happy to be back in Canada after 56 years away. Every day, he looked out the window and said how lucky he felt to be here.
>> Jim loved his family very much. He is survived by Christine, his wife of 43 years; his three children: Alice (Stephen Hoffmann), Emily (Roger Turner), and John; and his four grandchildren: Sam and Laura Turner, and Simon and Jane Hoffmann. A private funeral was held this week in Sechelt. All will be welcome to share stories about Jim at the celebration of his life on July 31, 2019 at the Sechelt Botanical Gardens (
https://coastbotanicalgarden.org). We are also hoping to arrange an event May 13 in Madison, time and location to be determined. People are also invited to send stories and photos to
[hidden email] to be bound into a book for his family. In lieu of flowers, donations can be made to the Sunshine Coast Community Solar Association:
https://suncoastcommsolar.weebly.com. His family will post updates on the memorial celebration and donation fund at
http://pawleypudding.ca/. ”
>>
>> Stephen H. Cody
>>
> --
> ------------------------------------------------------------
> Steffen Dietzel, PD Dr. rer. nat
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