Re: Power at Immersion Lenses

Posted by James Jonkman-2 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Power-at-Immersion-Lenses-tp7589305p7589307.html

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Hi, Claire.  I can think of 2 ways to do this.  You could measure the power with a 10x objective, and then compare the transmission curves from the manufacturer for the 10x and the 63 objectives and estimate the correction.  The transmission of the objective would be the main difference, but depending on how the back aperture of the objective is filled this isn't perfect.  Second way would be to get a Thorlabs power meter - they have a microscope slide shaped sensor which is designed to accommodate immersion media (model S170C).  I use it with the PM400 touch screen - it's an excellent combination and worth the investment.  I also use it to log power fluctuations - you can set the data logging as fast as every 10 ms to watch for faster fluctuations, or for longer durations I set it to measure every 60s and walk away for a couple of hours.

(no commercial interest)

Cheers,
James

-----------------------------------------------
   James Jonkman, Manager
   Advanced Optical Microscopy Facility (AOMF)
   University Health Network
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