Posted by
Craig Brideau on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Power-at-Immersion-Lenses-tp7589305p7589311.html
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As James mentioned, the S170C is designed for high NA oil and water
dipping/immersion lenses. Full disclosure Pina Colarusso and I helped Thor
with the prototype. If you use that one, the PM100D power meter is cheaper
than the PM400 and has similar logging features. Finally, don't directly
focus on the surface of the sensor, back away as much as practical without
causing the immersion medium to fall off the lens or 'funnel' too tightly.
You will get more accurate readings this way as you will be using more area
of the sensor and it tends to smooth out the reading.
Craig
On Fri, Mar 15, 2019 at 10:38 AM James Jonkman <
[hidden email]>
wrote:
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>
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>
> Hi, Claire. I can think of 2 ways to do this. You could measure the
> power with a 10x objective, and then compare the transmission curves from
> the manufacturer for the 10x and the 63 objectives and estimate the
> correction. The transmission of the objective would be the main
> difference, but depending on how the back aperture of the objective is
> filled this isn't perfect. Second way would be to get a Thorlabs power
> meter - they have a microscope slide shaped sensor which is designed to
> accommodate immersion media (model S170C). I use it with the PM400 touch
> screen - it's an excellent combination and worth the investment. I also
> use it to log power fluctuations - you can set the data logging as fast as
> every 10 ms to watch for faster fluctuations, or for longer durations I set
> it to measure every 60s and walk away for a couple of hours.
>
> (no commercial interest)
>
> Cheers,
> James
>
> -----------------------------------------------
> James Jonkman, Manager
> Advanced Optical Microscopy Facility (AOMF)
> University Health Network
> MaRS, PMCRT tower, 101 College St., Room 15-305
> Toronto, ON, CANADA M5G 1L7
>
[hidden email] Tel: 416-581-8593
> www.aomf.ca
>