Re: General question: Software vs. hardware

Posted by Glyn Nelson on
URL: http://confocal-microscopy-list.275.s1.nabble.com/General-question-Software-vs-hardware-tp7589316p7589325.html

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To throw my ha'penn'eth's worth in, I am on the side of confocal for a lot of the same reasons: no artefacts in the first place, plus deconvolution can be applied to confocal images for a minor improvement (and I believe is recommended in Pawley's excellent book).  

All of the things that would make a widefield system comparable (multiple cameras, fast GPUs, pixel shift free filtercubes etc) just bring the price of the widefield close to a confocal anyway.  Admittedly the running costs are cheaper though.

So to summarise- confocal + deconvolution is my preference.

Glyn