Posted by
Craig Brideau on
URL: http://confocal-microscopy-list.275.s1.nabble.com/General-question-Software-vs-hardware-tp7589316p7589336.html
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If you don't know how it works, you can't use it. There's always a chance
the features of your sample will fall on some edge case of the mystery
algorithm and give you BS results. If you can't prove the algorithm works
correctly on your samples it's not solid data, and if you don't know the
algorithm used then you can't do that.
Craig
On Mon, Mar 18, 2019 at 2:04 PM Csúcs Gábor <
[hidden email]>
wrote:
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>
> Connected to the same thread I would like your opinion about the new Leica
> davalopment (they call it "computational clearing" and sell it under the
> name "Thunder".
> They are very resistant to tell any mathematical details about it (even in
> a recently published white paper). Based on some rudimentaqry explanation
> it seems (really my subjective interpretation)that they do a wavelet
> conversion, filter out the large spatial components (claiming that those
> correspond to the out-of-focus background) and then transfer back the data.
> With this they claim to have the same effect as structured illumination
> (e.g. ApoTome) or a spinning disk (though this is a clear overstatement).
> Any thoughts from the community?
>
> Greetings Gabor
>