Well-liked 405 secondary antibodies

Posted by Joe Lebowitz on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Well-liked-405-secondary-antibodies-tp7589467.html

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Hello all,

We have had some reproducibly disappointing results with Alexa Fluor 405
tagged secondary antibodies.

Does anyone have a go-to secondary in this color other than the AF? We are
trying to multiplex as many channels as possible and this is the only one we
struggle to get good brightness/SNR with.

Thanks!

joe

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Joe Lebowitz
Ph.D. Candidate
NINDS Pre-doctoral Fellow
Khoshbouei Lab
UF Department of Neuroscience
[phone]