Re: Well-liked 405 secondary antibodies

Posted by Craig Brideau on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Well-liked-405-secondary-antibodies-tp7589467p7589468.html

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What ever happened to Brilliant Violet? I remember a bit of marketing about
it a while ago, but never got around to trying it myself. Did anyone get a
chance to give it a go?

Craig

On Thu, May 2, 2019 at 2:27 PM Joe Lebowitz <[hidden email]> wrote:

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> To join, leave or search the confocal microscopy listserv, go to:
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> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hello all,
>
> We have had some reproducibly disappointing results with Alexa Fluor 405
> tagged secondary antibodies.
>
> Does anyone have a go-to secondary in this color other than the AF? We are
> trying to multiplex as many channels as possible and this is the only one
> we
> struggle to get good brightness/SNR with.
>
> Thanks!
>
> joe
>
> --------------------------------
>
> Joe Lebowitz
> Ph.D. Candidate
> NINDS Pre-doctoral Fellow
> Khoshbouei Lab
> UF Department of Neuroscience
> [phone]
>
>
>