Re: Well-liked 405 secondary antibodies

Posted by kspencer007 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Well-liked-405-secondary-antibodies-tp7589467p7589469.html

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Hi Joe;
        I've had good success with Becton Dickinson BV dyes. BV421 is the best blue fluorescent dye I've tried.
        http://www.bdbiosciences.com/us/solrSearch?text=bv421&x=0&y=0

Kathy

The Scripps Research Institute
Dept of Molecular and Cellular Neuroscience
10550 N. Torrey Pines Road
DNC 216
La Jolla, Ca 92037





-----Original Message-----
From: Confocal Microscopy List <[hidden email]> On Behalf Of Joe Lebowitz
Sent: Thursday, May 02, 2019 1:17 PM
To: [hidden email]
Subject: Well-liked 405 secondary antibodies

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Hello all,

We have had some reproducibly disappointing results with Alexa Fluor 405 tagged secondary antibodies.

Does anyone have a go-to secondary in this color other than the AF? We are trying to multiplex as many channels as possible and this is the only one we struggle to get good brightness/SNR with.

Thanks!

joe

--------------------------------  

Joe Lebowitz
Ph.D. Candidate
NINDS Pre-doctoral Fellow
Khoshbouei Lab
UF Department of Neuroscience
[phone]