Posted by
Armstrong, Brian on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Well-liked-405-secondary-antibodies-tp7589467p7589470.html
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Hi Joe, we struggle with this also. I imagine it is a common complaint. Unfortunately, I would have to say that, imaging blue fluorophores with a 405nm LASER on a Confocal, we have had the best luck with Alexa 405.
Sorry,
Brian Armstrong PhD
Associate Research Professor
Developmental and Stem Cell Biology
Diabetes and Metabolic Diseases
Director, Light Microscopy Core
Beckman Research Institute, City of Hope
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Sent: Thursday, May 02, 2019 1:17 PM
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Subject: Well-liked 405 secondary antibodies
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Hello all,
We have had some reproducibly disappointing results with Alexa Fluor 405 tagged secondary antibodies.
Does anyone have a go-to secondary in this color other than the AF? We are trying to multiplex as many channels as possible and this is the only one we struggle to get good brightness/SNR with.
Thanks!
joe
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Joe Lebowitz
Ph.D. Candidate
NINDS Pre-doctoral Fellow
Khoshbouei Lab
UF Department of Neuroscience
(561) 504-3810
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