Re: Well-liked 405 secondary antibodies

Posted by Alison J. North on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Well-liked-405-secondary-antibodies-tp7589467p7589471.html

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Hi Joe,

I really like DyLight 405 both for confocal and 3D-SIM.  It's very picky
regarding anti-fades though - use Prolong Diamond (uncured) and it is
just fine (not so good with Prolong Gold). Just to warn you, if you look
down the microscope visually you will think the staining hasn't worked,
the autofluorescence overwhelms the dye, but when you start imaging it's
totally different.

Good luck!

Alison


On 5/2/2019 4:16 PM, Joe Lebowitz wrote:

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> Hello all,
>
> We have had some reproducibly disappointing results with Alexa Fluor 405
> tagged secondary antibodies.
>
> Does anyone have a go-to secondary in this color other than the AF? We are
> trying to multiplex as many channels as possible and this is the only one we
> struggle to get good brightness/SNR with.
>
> Thanks!
>
> joe
>
> --------------------------------
>
> Joe Lebowitz
> Ph.D. Candidate
> NINDS Pre-doctoral Fellow
> Khoshbouei Lab
> UF Department of Neuroscience
> [phone]
>
>  

--
Alison J. North, Ph.D.,
Research Associate Professor and
Senior Director of the Bio-Imaging Resource Center,
The Rockefeller University,
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