http://confocal-microscopy-list.275.s1.nabble.com/Well-liked-405-secondary-antibodies-tp7589467p7589474.html
BV421 is good but I always use it for highly expressed antigens. Also since it is a polymer dye it does not penetrate thick tissue sections well so some optimization needs to be done.
> On May 2, 2019, at 4:45 PM, Armstrong, Brian <
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> Hi Joe, we struggle with this also. I imagine it is a common complaint. Unfortunately, I would have to say that, imaging blue fluorophores with a 405nm LASER on a Confocal, we have had the best luck with Alexa 405.
> Sorry,
>
> Brian Armstrong PhD
> Associate Research Professor
> Developmental and Stem Cell Biology
> Diabetes and Metabolic Diseases
> Director, Light Microscopy Core
> Beckman Research Institute, City of Hope
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> -----Original Message-----
> From: Confocal Microscopy List [mailto:
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> Sent: Thursday, May 02, 2019 1:17 PM
> To:
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> Subject: Well-liked 405 secondary antibodies
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> Hello all,
>
> We have had some reproducibly disappointing results with Alexa Fluor 405 tagged secondary antibodies.
>
> Does anyone have a go-to secondary in this color other than the AF? We are trying to multiplex as many channels as possible and this is the only one we struggle to get good brightness/SNR with.
>
> Thanks!
>
> joe
>
> --------------------------------
>
> Joe Lebowitz
> Ph.D. Candidate
> NINDS Pre-doctoral Fellow
> Khoshbouei Lab
> UF Department of Neuroscience
> (561) 504-3810
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