Re: Well-liked 405 secondary antibodies **COMMERCIAL RESPONSE**

Posted by Jeffrey Carmichael on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Well-liked-405-secondary-antibodies-tp7589467p7589476.html

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Craig,

You've probably heard some of this from me, and I know that George McNamara
has also been impressed with and written of the Brilliant Horizon fluors
from BD (BD owns this from Sirigen purchase).

Here's a reference using Brilliant Violet 421 & Brilliant Violet 480:

"Multilocus Imaging of the E. coli Chromosome by Fluorescent In Situ
Hybridization."
https://www.researchgate.net/publication/319289637_Multilocus_Imaging_of_the_E_coli_Chromosome_by_Fluorescent_In_Situ_Hybridization

BV421 is approx. 20-40-fold brighter than AF405. BV480 is also approx.
25-fold brighter than CFP (depending on who is measuring...)

The problem remains the availability of reagents, as BD has decided that
their business model is better served by reserving these reagents for their
custom-conjugated Ab's for flow.....But I believe that BD still entertains
custom requests for their reagents.

IMHO, directly conjugated, primary Ab's are the best way to leverage these
fluors because they're so bright that they don't need amplification. This
removes the problem of species specificity....Why wouldn't one try this???

Here's a link to a presentation that illustrates the efficacy of this
approach of filling the gap of violet & blue fluors.  Given the headlong
stampede towards increased multiplexing, I don't understand how these
fluors could be overlooked:
https://www.chroma.com/5-channel-fluorescence-imaging-simplified

I hesitate using the "COMMERCIAL RESPONSE" qualifier, because Chroma
Technology has no formal relationship with BD Biosciences and receives no
credit for purchases of related products. Chroma Technology profits only
from sales of filter sets which are used to image these fluors.

Jeff

*Jeff Carmichael*

*Director of Marketing*

*[hidden email] <[hidden email]> | 802-428-2528*



On Thu, May 2, 2019 at 4:47 PM Craig Brideau <[hidden email]>
wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> What ever happened to Brilliant Violet? I remember a bit of marketing about
> it a while ago, but never got around to trying it myself. Did anyone get a
> chance to give it a go?
>
> Craig
>
> On Thu, May 2, 2019 at 2:27 PM Joe Lebowitz <[hidden email]> wrote:
>
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > Post images on http://www.imgur.com and include the link in your
> posting.
> > *****
> >
> > Hello all,
> >
> > We have had some reproducibly disappointing results with Alexa Fluor 405
> > tagged secondary antibodies.
> >
> > Does anyone have a go-to secondary in this color other than the AF? We
> are
> > trying to multiplex as many channels as possible and this is the only one
> > we
> > struggle to get good brightness/SNR with.
> >
> > Thanks!
> >
> > joe
> >
> > --------------------------------
> >
> > Joe Lebowitz
> > Ph.D. Candidate
> > NINDS Pre-doctoral Fellow
> > Khoshbouei Lab
> > UF Department of Neuroscience
> > [phone]
> >
> >
> >
>

--
 <https://www.chroma.com/>CHROMA TECHNOLOGY CORP®
*an employee owned
company*
10 Imtec Lane, Bellows Falls, Vermont 05101 USA
800-824-7662 |
FAX: [phone]
www.chroma.com <https://www.chroma.com/> | 
[hidden email] <mailto:[hidden email]>