Posted by
Tim Feinstein on
URL: http://confocal-microscopy-list.275.s1.nabble.com/High-Content-Equipment-tp7589531p7589533.html
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I think you need to specify the scale of your project. The easiest answer is to go with a stage incubator that accommodates multiwell plates. A 96-well dish, automated focus control and some experience with R, CellProfiler and ImageJ scripting will get you quite a lot of throughput. All of the major scope companies can do this or you can get custom solutions from, e.g., Andor, or even Thorlabs if you are ready to help build & maintain it. On the other hand I don't know of many stage-top incubators that will let you do fluid handling in a 24- or 96-well dish like a plate reader would; Thorlabs _might_ have some ideas about that.
In general if you need multiwell fluid handling, +/- automated plate exchange, AND a warranty, you may need a "big box" high-content imager. A good list of available options, both widefield and spinning disc, starts on page 558 of the Lilly Assay Guidance Manual:
https://www.ncbi.nlm.nih.gov/books/NBK53196/Best,
T
Timothy Feinstein, Ph.D.
Research Scientist
Department of Developmental Biology
University of Pittsburgh
On 5/19/19, 5:24 AM, "Confocal Microscopy List on behalf of Pedro Camello" <
[hidden email] on behalf of
[hidden email]> wrote:
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Hi all,
I would appreciate experiences/opinion for the purchase of a HC imaging system. The goal is work with live cultures, in a variety of formats, and ability for liquid addition would be desirable. Confocal would be a secondary ability.
Many thanks for your help