Posted by
Pat Robison on
URL: http://confocal-microscopy-list.275.s1.nabble.com/SSD-RAID-for-new-wide-field-microscopy-system-tp7589536p7589541.html
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While it can be hard to predict exactly what form new experiments will
take, I assume that you are buying a system for live imaging time lapse
because you or another user has a particular process in mind that you want
to image. It helps to make some back of the envelope type calculations- if
the timescale of my process is x seconds, I usually want 2-3 frames every x
seconds for minimalist capture, up to maybe 10 frames every x seconds if I
want kinetics. That capture rate, stack size (given by the physical
dimensions of your sample and the z diffraction limit), and the expected
time you can keep a sample on the scope gives you a good maximum estimate
of how many frames to expect, and therefore file size. I would echo what
others have said about the SSD though- 6k is an awful lot of money for that
much drive, and there is not much benefit to having a RAID be solid state
to begin with. If possible I would upgrade myself with a normal RAID for a
fraction of the cost.
-Pat Robison
On Thu, May 23, 2019, 9:48 AM Mirco Martino <
[hidden email] wrote:
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> To join, leave or search the confocal microscopy listserv, go to:
>
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy> Post images on
http://www.imgur.com and include the link in your posting.
> *****
>
> Hi all,
>
> we are buying a new fluorescent microscope wide-field system. The system
> include a workstation with 64GB DDR4 RAM, 11GB of graphic card, 512 gb of
> memory and SSD-RAID of 2TB. There is an option to increase the SSD-RAID to
> 4TB for about 6000$ extra. The system will be use for both fixed materiel
> imaging and for live-imaging with time-laps, z-stack, tiling and
> multi-fluorophores experiments. I was wondering if it is worth to invest
> those extra money for this option or not. This will be the first time we
> will have time-laps experiments, so I don't know what to expect as pictures
> size.
>
> What do you think?
>
> Thanks in advance for your comments and opinions!
>