spectral unmixing with Zeiss 880
Posted by
Cammer, Michael on
URL: http://confocal-microscopy-list.275.s1.nabble.com/spectral-unmixing-with-Zeiss-880-tp7589728.html
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We are trying to do spectral unmixing with a series of Opal probes with the Zeiss 880 spectral detector. We have collected the spectra of each one and saved them.
Question: for each image that we want to use these references for, how can we automatically populate the unmixing settings with these. We need to load six spectra and have the LUTs set in a standard manner.
We tried loading them and setting the LUTs, then saving the image we were using them with, and reusing the settings, but the spectra profile do not get propagated. We cannot set six spectra and their LUTs manually for every image that we need to unmix.
Help with this greatly appreciated.
Thank you.
Michael Cammer, Sr Research Scientist, DART Microscopy Laboratory
NYU Langone Health, 540 First Avenue, SK2 Microscopy Suite, New York, NY 10016
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