Re: spectral unmixing with Zeiss 880

Posted by G. Esteban Fernandez on
URL: http://confocal-microscopy-list.275.s1.nabble.com/spectral-unmixing-with-Zeiss-880-tp7589728p7589737.html

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What I do is open an unmixed image (i.e. resulting channel image not lambda
stack) and click Read Parameters From Image under Processing > Linear
Unmixing.

-Esteban

On Wed, Aug 7, 2019 at 8:10 AM Cammer, Michael <
[hidden email]> wrote:

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> We are trying to do spectral unmixing with a series of Opal probes with
> the Zeiss 880 spectral detector.  We have collected the spectra of each one
> and saved them.
>
>
> Question:  for each image that we want to use these references for, how
> can we automatically populate the unmixing settings with these.  We need to
> load six spectra and have the LUTs set in a standard manner.
>
>
> We tried loading them and setting the LUTs, then saving the image we were
> using them with, and reusing the settings,  but the spectra profile do not
> get propagated.  We cannot set six spectra and their LUTs manually for
> every image that we need to unmix.
>
>
> Help with this greatly appreciated.
>
>
> Thank you.
>
>
> Michael Cammer, Sr Research Scientist, DART Microscopy Laboratory
>
> NYU Langone Health, 540 First Avenue
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