Re: CO2 enrichment accessories

Posted by daj1u06 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/CO2-enrichment-accessories-tp7589882p7589887.html

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Hi Anna, we just 3D print new holders and covers for any type of dish in plastic (PLA) with thin glass lids (either extra large coverslips or extra large microscope slides (patholgy labs have these) held in with silicone) as we need them. Happy to share CAD files with anyone who wants them.

Tubing connectors, go to Cole Palmer web site and look for leur fittings, they have 100s of variants available.

 For multiwall plates, we use a wide bore (cream hub, 19G) hypodermic needle  - heat one to red heat and insert through side wall immediately below lid in middle of short end. Withdraw and reheat and then push back through first hole and through first dam between wells. Replace with clean needle and bend needle slightly so plate can fit into carrier snuggly without the needle lifting one end up. Coming in from side rather than top avoids any issues with condensor. we tend to tape the gas delivery tubing onto the stage to prevent needle moving through tubing flex as stage moves

Regards,

DAJ
Dave Johnston
Light Microscopy Manager,
Biomedical Imaging Unit,
Faculty of Medicine, University of Southampton and University Hospital Southampton NHS Foundation Trust

MP12, Level B, Lab and Path Block, University Hospital Southampton, Tremona Road, Southampton, SO16 7LS.
tel: [phone]
email: [hidden email]