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Sylvie Le Guyader on
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Hi again
We never use any training sample. The reason why our training is so long (and expensive: 1200€) is because we troubleshoot the sample preparation and experimental design extensively before and during (and after) the training.
After our first 1h meeting briefing the researcher on how to modify their sample if it is needed (most of the time but not always), we demand that they bring the correct samples during the training.
It is very rare that they don’t and come up with samples that they got from a colleague. If they do we cancel the training and send them back to the bench.
Most of them understand after our 1h meeting that it is fully in their interest to get the best samples possible during the training because we help them acquire data during the week.
Our trainings are mandatory 1 to 1. We do not train people together. Therefore the training is always adapted to their sample/experiment/experience/personality...
My colleague Gabriela Imreh does all the trainings. She squeezes about 40 trainings per year outside of holidays. And our microscopy course takes 1 full month during which there is no training.
Keeps us seriously interested and busy! 😉
Med vänlig hälsning / Best regards
Sylvie
@@@@@@@@@@@@@@@@@@@@@@@@
Sylvie Le Guyader, PhD
Live Cell Imaging Facility Manager
Karolinska Institutet- Bionut Dpt
Blickagången 16,
Room 7362 (lab)/7840 (office)
14157 Huddinge, Sweden
mobile: +46 (0) 73 733 5008
LCI website
Follow our microscopy blog!
-----Original Message-----
From: Confocal Microscopy List <
[hidden email]> On Behalf Of Arvydas Matiukas
Sent: 16 October 2019 17:46
To:
[hidden email]
Subject: Re: [EXTERNAL] Re: Confocal and Widefield microscopy training material
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Hi Silvie,
That's useful approach to try. Whenever possible I try an individual approach matching the scope and depth of the material to the trainee's background and skill level. The worst scenario is when a lab sends in several trainees of different level (e.g. student, technician and postdoc). Do you accept several trainees for the same session?
Next major difficulty for the trainee that I noticed is the transition from imaging training sample to the self-prepared one. Here the ability to duplicate tutorial actions usually is not enough , in depth understanding is required, and of course preparing quality samples.
Best regards,
Arvydas
>>> Sylvie Le Guyader <
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[hidden email]>> 10/16/2019 9:54 AM >>>
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’ significant fraction of the basic confocal trainees bored after 1-2 hrs of total 3.’
Definitely! That would bore me too and I am not from the millennium generation! I believe that the only way is to make very short videos and give quizzes half way or just afterwards. E.g. our trainee watches our video about bit depth, saturation and under exposure knowing that right after the video we will ask to show us where the buttons are and if there was any saturation/under exposure in the image they took immediately before watching the video. They are encouraged to stop the video and push the buttons on the software whenever they feel like it.
Med vänlig hälsning / Best regards
Sylvie
@@@@@@@@@@@@@@@@@@@@@@@@
Sylvie Le Guyader, PhD
Live Cell Imaging Facility Manager
Karolinska Institutet- Bionut Dpt
Blickagången 16,
Room 7362 (lab)/7840 (office)
14157 Huddinge, Sweden
mobile: +46 (0) 73 733 5008
LCI
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From: Arvydas Matiukas <
[hidden email]<mailto:
[hidden email]>>
Sent: 16 October 2019 13:53
To: Sylvie Le Guyader <
[hidden email]<mailto:
[hidden email]>>;
[hidden email]<mailto:
[hidden email]>
Subject: Re: [EXTERNAL] Re: Confocal and Widefield microscopy training material
Hello Sylvie,
Thanks for pointing to these video tutorials by Alyona Minina. They nicely complement traditional powerpoint material and hopefully could save some my time. I am curious to check how much longer video material keeps trainee's attention focussed, especially for millenial youtube generation. I noticed that traditional verbal and powerpoint presentation makes significant fraction of the basic confocal trainees bored after 1-2 hrs of total 3.
with best wishes Arvydas
+++++++++++++++++++++++++++++
Arvydas Matiukas, Ph.D.
Manager of NRB Shared Equipment
Director of Confocal&Two-Photon Core
SUNY Upstate Medical University
>>> Sylvie Le Guyader
<
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[hidden email]<mailto:
[hidden email]%3cmailto:
[hidden email]>>> 10/16/19 6:28 AM >>>
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Hi Niyanta
Alyona Minina has tons of videos on you tube. Check her channel<
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Med vänlig hälsning / Best regards
Sylvie
@@@@@@@@@@@@@@@@@@@@@@@@
Sylvie Le Guyader, PhD
Live Cell Imaging Facility Manager
Karolinska Institutet- Bionut Dpt
Blickagången 16,
Room 7362 (lab)/7840 (office)
14157 Huddinge, Sweden
mobile: +46 (0) 73 733 5008
LCI website
Follow our microscopy blog!
-----Original Message-----
From: Confocal Microscopy List
<
[hidden email]<mailto:
[hidden email]<mailto:
[hidden email]%3cmailto:
[hidden email]>>>
On Behalf Of Niyanta Kumar
Sent: 11 October 2019 22:00
To:
[hidden email]<mailto:
[hidden email]<mailto:
[hidden email]%3cmailto:
[hidden email]>>
Subject: Confocal and Widefield microscopy training material
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Hi all,
Can someone suggest any existing resources and formats to pull from for designing new and advanced user trainings for confocal and widefield microscopy? I need to design trainings for a Zeiss LSM 980 Airyscan 2 confocal and an Axio Obsever widefield scope. Interactive resources or existing quizzes that can ensure users know what they need to would also be helpful.
Thanks,
Niyanta
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https://eur01.safelinks.protection.outlook.com/?url=https%3A%2F%2Furldefense.proofpoint.com%2Fv2%2Furl%3Fu%3Dhttps-3A__ki.se_medarbetare_integritetsskyddspolicy%26d%3DDwIGaQ%26c%3Dogn2iPkgF7TkVSicOVBfKg%26r%3DDtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k%26m%3DXGh1Uu87TWxekpsyPOgE9NKlXFtrItrNJpVgKlIIhEk%26s%3D8j7d7fhNJQoj3Rai2nIAydL3Me-74TRwHI-43to-b0M%26e&data=02%7C01%7Csylvie.le.guyader%40KI.SE%7C4b1fe493ee6a493a196208d752500e40%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C637068376112645232&sdata=PpGGWwGCBcwcu8Zbs2PWoU1aN6HPOvdkeBbI2z51e7U%3D&reserved=0=>.
Sending email to Karolinska Institutet (KI) will result in KI processing your personal data. You can read more about KI’s processing of personal data here<
https://eur01.safelinks.protection.outlook.com/?url=https%3A%2F%2Furldefense.proofpoint.com%2Fv2%2Furl%3Fu%3Dhttps-3A__ki.se_en_staff_data-2Dprotection-2Dpolicy%26d%3DDwIGaQ%26c%3Dogn2iPkgF7TkVSicOVBfKg%26r%3DDtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k%26m%3DXGh1Uu87TWxekpsyPOgE9NKlXFtrItrNJpVgKlIIhEk%26s%3DqzKolo6QFEOyQO9RlKOPvFp7_-GjfPYp-nmKl8nobas%26e&data=02%7C01%7Csylvie.le.guyader%40KI.SE%7C4b1fe493ee6a493a196208d752500e40%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C637068376112655227&sdata=l4bq27kVb9mYVZVjJZo%2BkXxQb4VG6LNEUSAmzW9toBs%3D&reserved=0=>.
När du skickar e-post till Karolinska Institutet (KI) innebär detta att KI kommer att behandla dina personuppgifter. Här finns information om hur KI behandlar personuppgifter<
https://ki.se/medarbetare/integritetsskyddspolicy>.
Sending email to Karolinska Institutet (KI) will result in KI processing your personal data. You can read more about KI’s processing of personal data here<
https://ki.se/en/staff/data-protection-policy>.