Posted by
Arvydas Matiukas on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Confocal-and-Widefield-microscopy-training-material-tp7589972p7590012.html
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Hi Steffen, Silvie
Thanks for very interesting and useful discussion. Few summary
thoughts:
1. I second Steffens' reasoning for starting training with Core
provided=known=calibrated samples. Sylvie's approach of starting with
users'=non calibrated sample introduces to many unknowns at a time and
to my opinion reduces efficiency of the training. I personally prefer
not to be trained in such stressful way.
2. I wonder if many labs in EU can afford to spend 1 month of their
member time and pay 1200EUR to get the members trained in advanced
level microscopy. In the USA it definitely not going to happen on any
significant scale.
Maybe a cultural difference :) I recall the comparison provided by US
based Zeiss specialist after his training in Germany: "problem=
ballpoint pen stopped writing. German style solution = research
potential causes, i.e. disassemble the pen, check if any ink is left, if
ball rotates freely, finally if the user properly presses knob or
rotates the body. This approach may take significant but reasonable time
and provide useful skills and experience for the future. American style
solution = check the pen price first. As it is much lower than the fee
of professional spending hours on this problem then just trash the pen
and get a new one"
3. Of course in the USA there are labs that need advanced level
microscopy. However, typically they have their own microscopes and
microscopy experts and very rarely use Core services.
Among my 400 trainees over 15 years only about 10 expressed willingness
to proceed to the advanced level, and among them only 1 grad student.
4. Finally, time and cost considerations. At 1 month and 1200EUR
spending level how many techniques a beginner researcher may learn in
1 year ? 3years? BTW I think training in optical/confocal microscopy is
relatively inexpensive, preparing your own samples typically is more
time consuming and expensive, and learning other tecniques may be more
expensive as well.
My 2 cents,
Arvydas
+++++++++++++++++++++++++++++
Arvydas Matiukas, Ph.D.
Manager of NRB Shared Equipment
Director of Confocal&Two-Photon Core
SUNY Upstate Medical University
Neuroscience & Physiology Dept
>>> Steffen Dietzel <
[hidden email]> 10/17/2019 6:55 AM >>>
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Hi Niyanta,
I have collected a few resources on our website, so that I can refer
our
users to it more easily. This is at
https://urldefense.proofpoint.com/v2/url?u=https-3A__www.bioimaging.bmc.med.uni-2Dmuenchen.de_learn_&d=DwIDaQ&c=ogn2iPkgF7TkVSicOVBfKg&r=DtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k&m=99s_HGKyJaDs8OsbjeTtnUeJkyXm5p5eS4vds5qQbM0&s=Z2jynrNg35the3m1H5X3AsUA52lnDqOb4qZUaoY05dY&e=Have a look at "Educational Websites" and "Materials for teaching".
Maybe you find something helpful. The listed page from Australia (was
mentioned by others too) contains a quiz that you can take, and you can
print out a certificate if you answer 8+ of 10 questions correct.
As in terms of hands on training, we start with a 3 h introduction
session for up to 3 people. Usually with our samples, for two reasons.
First, we want the user to pay attention to the microscope and
software,
not on the biological content of the images we make. Second, this way
we
can be sure that the sample is all right and we don't have users with
crappy samples blaming it on the microscope. I agree with others that
after 3 hours it makes no more sense, brains are full.
Next we do a 'guided session' with the user's sample that we accompany
until the user is getting good results. This is 1:1. Depending on
sample
difficulty, prior knowledge and user talent we sometimes have a third
session.
I am impressed by Sylvie's approach, but I doubt our group leaders
would
be willing to come up with so much money for training. Some think what
we do now is already too time consuming (but most are happy about it).
Steffen
Am 11.10.2019 um 21:59 schrieb Niyanta Kumar:
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> Hi all,
> Can someone suggest any existing resources and formats to pull from
> for designing new and advanced user trainings for confocal and
> widefield microscopy? I need to design trainings for a Zeiss LSM 980
> Airyscan 2 confocal and an Axio Obsever widefield scope. Interactive
> resources or existing quizzes that can ensure users know what they
> need to would also be helpful.
> Thanks,
> Niyanta
>
--
------------------------------------------------------------
Steffen Dietzel, PD Dr. rer. nat
Ludwig-Maximilians-Universität Mnchen
Biomedical Center (BMC)
Head of the Core Facility Bioimaging
Großhaderner Straße 9
D-82152 Planegg-Martinsried
Germany
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