Posted by
Alison J. North on
URL: http://confocal-microscopy-list.275.s1.nabble.com/white-light-laser-tp7590046p7590047.html
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Hi Konstantin,
I am not aware of any other company than Leica offering a WLL on a
confocal microscope (LaVision/Miltenyi offers it on their light sheet
system too, for example), but I may well be wrong. The WLL is fantastic
- very flexible, allows you to go down to pretty long wavelengths (670
nm, unless that has changed now), and also enables you to do time gating
in conjunction with the HyDs. It does have the disadvantage of not
being so powerful at a particular selected wavelength - for imaging we
rarely found that to be an issue, as you can always activate two lines
close together to increase the power, but it is a problem if you want to
do FRAP. One other potential disadvantage is that if the WLL goes down,
you have lost all of your lasers in effect, but our experience has been
that Leica are pretty efficient at replacing it, though obviously
service reliability varies hugely with geographical location.
All the best,
Alison
On 10/21/2019 9:28 AM, Konstantín Levitskiy wrote:
--
Alison J. North, Ph.D.,
Research Associate Professor and
Senior Director of the Bio-Imaging Resource Center,
The Rockefeller University,
1230 York Avenue,
New York,
NY 10065.
Tel: office ++ 212 327 7488
Tel: lab ++ 212 327 7486
Fax: ++ 212 327 7489