Posted by
Jacqueline Ross on
URL: http://confocal-microscopy-list.275.s1.nabble.com/white-light-laser-tp7590046p7590050.html
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Hi Konstantin,
I am also very interested in the advantages and disadvantages of the WLL. Our systems all have 4-5 lasers that cover the visible spectrum well (405 - 635nm). Given that most fluorophores don't have a very narrow absorption spectrum, how important is the flexibility of the WLL? I think it would be very useful perhaps for spectral unmixing but how often do users change the excitation when using the WLL?
The only laser line that I miss having now and which would be great to have on any new system is a UV. We recently decommissioned our last confocal which had the water-cooled argon with 351/364nm. I was really sad to see it go.
Cheers,
Jacqui
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From: Confocal Microscopy List [
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Sent: 22 October 2019 02:28
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Subject: white light laser
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Dear Microscopists,
Do you know if any other company, besides Leica, include white light laser
in their confocals. Or if any other technology can replicate the free
selection wavelength laser light? On the other hand, what are disadvantages
of the WLL?
Best regards,
Dr. Konstantín Levitskiy
Servicio de Microscopía
InstitutodeBiomedicinadeSevilla - IBiS
Email: <mailto:
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Web: <
http://www.ibis-sevilla.es/> www.ibis-sevilla.es