Re: white light laser

Posted by Craig Brideau on
URL: http://confocal-microscopy-list.275.s1.nabble.com/white-light-laser-tp7590046p7590052.html

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I have also noticed fewer options for NUV sources for microscopy. The last
time we tried it I had to take the extreme step of frequency-doubling a
Ti:Saph connected to our multiphoton/confocal system and re-routing the
resulting 380nm beam through the confocal optics. It was a very crude
solution, but the Ti:Saph could be tuned and doubled from 350-540nm with a
crystal, then fiber-coupled for delivery to the confocal portion of the
scan head. I was mainly limited by the coatings on our optics or glass
absorption, as there was a significant drop-off of transmission at
wavelengths shorter than 375nm with the lens we were using.

https://spie.org/Publications/Proceedings/Paper/10.1117/12.2079625?SSO=1

Craig

On Mon, Oct 21, 2019 at 2:11 PM Jacqueline Ross <[hidden email]>
wrote:

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>
> Hi Konstantin,
>
> I am also very interested in the advantages and disadvantages of the WLL.
> Our systems all have 4-5 lasers that cover the visible spectrum well (405 -
> 635nm). Given that most fluorophores don't have a very narrow absorption
> spectrum, how important is the flexibility of the WLL? I think it would be
> very useful perhaps for spectral unmixing but how often do users change the
> excitation when using the WLL?
>
> The only laser line that I miss having now and which would be great to
> have on any new system is a UV. We recently decommissioned our last
> confocal which had the water-cooled argon with 351/364nm. I was really sad
> to see it go.
>
> Cheers,
>
> Jacqui
> ________________________________________
> From: Confocal Microscopy List [[hidden email]] on
> behalf of Konstantín Levitskiy [[hidden email]]
> Sent: 22 October 2019 02:28
> To: [hidden email]
> Subject: white light laser
>
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>
> Dear Microscopists,
>
> Do you know if any other company, besides Leica, include white light laser
> in their confocals. Or if any other technology can replicate the free
> selection wavelength laser light? On the other hand, what are disadvantages
> of the WLL?
>
> Best regards,
>
> Dr. Konstantín Levitskiy
>
> Servicio de Microscopía
>
> InstitutodeBiomedicinadeSevilla - IBiS
>
> Email:  <mailto:[hidden email]> [hidden email]
>
> Web:  <http://www.ibis-sevilla.es/> www.ibis-sevilla.es
>