Posted by
Darran Clements on
URL: http://confocal-microscopy-list.275.s1.nabble.com/white-light-laser-tp7590046p7590061.html
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Hi Craig
The differentiator here is on the illumination side as opposed to the detection side of the instrument. The Leica AOBS beamsplitter allows free selection of illumination lines. Multispectral parallel detection would potentially be as effective as the broadband spectrophotometer arrangement Leica uses as a detection system if all else was roughly equal, but Zeiss and the Nikon multichannel PMT as well as the spectrophotometer arrangement in the Fluoview are not supported with the AOBS, their filter beamsplitters mean that the flexible illumination options inherent in a supercontinuum white light laser can't be realised to the extent the AOBS makes possible. Each system has its own strengths and weaknesses, but the AOBS was a great innovation, and is an excellent fit for the white light laser.
Darran Clements
Imaging Facility Assistant
Wellcome-MRC Stem Cell Institute
Jeffrey Cheah Biomedical Centre
Cambridge Biomedical Campus
University of Cambridge
Puddicombe Way
Cambridge
CB2 0AW
[hidden email]
01223 760 201
-----Original Message-----
From: Confocal Microscopy List <
[hidden email]> On Behalf Of Craig Brideau
Sent: 22 October 2019 16:44
To:
[hidden email]
Subject: Re: white light laser
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Your point is very valid, Juan, which is why Leica typically offers this with their adjustable band 5-channel detector system. It is essentially a 5-band monochrometer system that can be adjusted to match the lines you have selected from the WLL. Zeiss also has a spectral offering that can do much the same thing, although I am not sure if it is as good at blocking the excitation as I have never personally used or observed it.
Craig
On Tue, Oct 22, 2019 at 3:21 AM Juan Luis Ribas <
[hidden email]> wrote:
> *****
> To join, leave or search the confocal microscopy listserv, go to:
>
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy> Post images on
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> *****
>
> Dear Konstantin,
>
> Zeiss can mount a white laser in their confocal but for me it has no
> sense to mount a white laser in a dichoric system. At the end, you are
> limited by the spliters.
>
> Best regards
>
> Juan Luis
>
>
> El 21/10/2019 a las 15:28, Konstantín Levitskiy escribió:
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> >
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy> > Post images on
http://www.imgur.com and include the link in your
> posting.
> > *****
> >
> > Dear Microscopists,
> >
> > Do you know if any other company, besides Leica, include white light
> laser
> > in their confocals. Or if any other technology can replicate the
> > free selection wavelength laser light? On the other hand, what are
> disadvantages
> > of the WLL?
> >
> > Best regards,
> >
> > Dr. Konstantín Levitskiy
> >
> > Servicio de Microscopía
> >
> > InstitutodeBiomedicinadeSevilla - IBiS
> >
> > Email: <mailto:
[hidden email]>
[hidden email]
> >
> > Web: <
http://www.ibis-sevilla.es/> www.ibis-sevilla.es
> >
> >
> > .
> >
> --
> /Juan Luis Ribas/
> Servicio de Microscopía
> Centro de Investigación, Tecnología e Innovación Universidad de
> Sevilla Av. Reina Mercedes 4b
> 41012 Sevilla
> Spain
>