Posted by
Mike Nelson on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Olympus-SLIDEVIEW-VS200-tp7590128p7590133.html
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Hi Andrew,
I have been looking into the Polaris, and was wondering if you could
elaborate on your experiences with it, if you have time? I believe the
current reason we have a lab interested in upgrading from the Vectra3 is
speed (Polaris claims 7 colors in 20 minutes, though for a small square),
but I also noticed that it claimed separation of 9 channels. Have you had
any issues with the color separation? I noticed that for the Vectra fields
of view on InForm, the color separation could be done tile by tile, or run
across a whole slide in the same way. I found issues with either method.
Unmixing is tricky business, and I'm wondering if the Polaris system has
made any improvements there. Most importantly, I do like my flexibility,
and tend to "get creative" with what can be done on the various confocal
systems we have. What limitations does the Polaris have that made you
consider it less flexible? And has service been good, if you have needed it?
Hi Dave,
Those quad cubes can be dangerous! :) Had "an experience" with the quad
cube from a Z1 at another institute. Helped me design tools to look at
colocalization and correlation between channels, though.
I too was impressed with the 3D histech systems (speed was great for both
BF and IF on the 250), though I did not like the variable
exposure/contrasting in the BF images. I could see in my QuPath analysis
pipelines where each tile edge was due to variation in the measured OD of
my cells (hopefully there is a setting where this could be turned off).
Also, I would recommend being very careful about the fluo images as the
mrxs format has compatibility issues. So unless want to do all of your
fluor analysis within 3DHISTECH's software, you might be in for a bit of
pain. Some notes from the open source community on that topic:
MRXS:
https://blog.openmicroscopy.org/file-formats/community/2016/01/06/format-support/More general:
https://blog.openmicroscopy.org/community/file-formats/2019/06/25/formats/https://forum.image.sc/t/ome-s-position-regarding-file-formats/26952Cheers,
Mike
On Fri, Nov 8, 2019 at 8:17 PM Andrew Woolley <
[hidden email]> wrote:
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>
> Hi Agnes,
>
> You will have to rank-order what are the most important features for
> your needs (z-stacking, automated scanning of 10s of slides or 100s of
> slides, 4 fluorescent channels, multi-user friendly, fast overview of
> whole slides, etc.) because no system does everything.
>
> I have used a number of top-of-the-line fluorescent slide scanners.
> Here are some options you may want to follow up on with providers:
>
> Vectra Polaris (our current workhorse in the lab) - user friendly, very
> fast, no z-stack, not very flexible (pretty locked-down in order to make
> it user friendly).
>
> Zeiss AxioScan Z1 slide scanner - like the Polaris it also takes slides
> in holders, and like the Polaris can scan many sides at a time, Zeiss
> has software to co-register across platforms, which may be very helpful
> for your specific need of matching a position from a slide scan to a
> more detailed confocal stack.
>
> Olympus SlideView VS200... this went on sale very recently (press
> release Oct 31st 2019 -
>
https://www.olympus-global.com/news/2019/nr01430.html ) so I don't think
> many have had a chance to use it, but it is similar in format to the
> Zeiss AxioScan Z1.
>
> Keyence BZX-810 - I'm including this because it has a holder for imaging
> 3 microscope slides at a time, and you can take 4-color fluorescent
> z-stacks across entire slides quickly if at low magnification (like
> 4x). Its also very flexible to do a number of other things, and accepts
> a variety of microscope objectives. Its relatively user friendly, and
> you only mentioned needing to image 'half a dozen slides', ... the
> systems above are for scanning in slides at much larger volumes and are
> maybe not what you need in the end unless you're digitizing every
> section through whole rodent brains. You could also buy 3 of these for
> the price of any of the others on the list. BUT its not strictly a
> slide scanner, and will not be performing the task of scanning slides
> nearly as fast or as easily on this system vs. the others on this list.
>
> Cheers,
>
> Andrew
>
> On 11/8/2019 10:01 AM, Craig Brideau wrote:
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> >
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy> > Post images on
http://www.imgur.com and include the link in your
> posting.
> > *****
> >
> > My own observation with several models of slide scanner is that sample
> > preparation and handling is probably more important than the equipment
> > itself. Most scanners use some flavor of autofocus, and the method used
> may
> > or may not be compatible with the various quirks of your specific
> samples.
> > If you are using a multi-slide handling system, the type of slides you
> use,
> > or any treatment during your process may literally gum up the feed
> > mechanism that transfers slides from storage to the imaging area. So in
> > short, you really won't know how a system is going to perform until you
> try
> > your specific samples on it. If you do get any advice from forum members,
> > I'd also ask exactly how they prepare their samples and how the system
> > handles things like misaligned coverslips and errant smudges.
> >
> > Craig
> >
> > On Fri, Nov 8, 2019 at 10:32 AM Agnes Janoshazi <
> >
[hidden email]> wrote:
> >
> >> *****
> >> To join, leave or search the confocal microscopy listserv, go to:
> >>
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy> >> Post images on
http://www.imgur.com and include the link in your
> posting.
> >> *****
> >>
> >> Hi,
> >> Need some god feedback.
> >> We are looking for the best slide viewer in the market. We have very
> short
> >> deadline.
> >> We need to screen whole brain tissues with 3-4 fluorescence labels.
> >> We need Z stack, and a fast overview of whole brain slice. We would like
> >> to image half dozen slides , get an overview image of each slide to
> >> assess them for further confocal study.
> >> We need an easy system for multi-user group.
> >> Please give us a feedback on Olympus SLIDEVIEW™ VS200 or better
> version.
> >> Thanks,
> >> Agnes
> >>
>