Re: Software for 3D and 4D analysis

Posted by Gary Laevsky on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Software-for-3D-and-4D-analysis-tp7590134p7590136.html

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We ended up going with Arivis.  It's the only software that didn't crash on
our multi TB datasets.

I also must say, the support from Arivis has been really good as well.

It's expensive though ...

As I'm sure you know (and has been said), demo demo demo ...

On Wed, Nov 13, 2019 at 11:05 AM Mike Nelson <[hidden email]> wrote:

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>
> We ended up going with Imaris, but I strongly recommend getting a free demo
> (they all should make one available) for your particular institute's area
> of interest or user model. There is usually a tradeoff between amount of
> options in terms of workflows and user interface accessibility/learning
> curve, so some of the choice may come down to whether the software is being
> dropped in the user's lap, or the core facility is running the analysis or
> guiding them through it. I have only demoed Arivis, and while the
> interactive 3D manipulation of volumes was neat, it was limited to a 512^3
> pixel volume at the time, which wasn't going to cut it as a useful tool.
> The one thing I *really *liked about Arivis that Imaris didn't have was the
> capacity to produce the 360 degree videos (search it on YouTube). It's the
> closest you can get right now to easily sharing a 3D volume, and can make
> for a great social media tool or intro to a lab's website. Imaris also
> doesn't seem to have much in the way of machine learning or deep learning
> algorithms currently built in (paint objects, find all objects that look
> like the painted objects), though you could build your own with MATLAB.
>
> In the end, we went with Imaris due to the workflow and ease of use for the
> core facility customers. It could be used for fairly complex analyses
> with *relatively
> *little training and has a reasonably clean user interface. Most commercial
> software can now handle very large images these days, but there is a
> difference between "handles" and "can populate with objects or do image
> analysis on," which was something we ran into with Amira. It could load the
> images, but you couldn't actually analyze the full volume (at the time).
>
> I haven't tried Aivia.
>
> The computer you want to run these programs on can also have a significant
> impact on whether they are actually useful to you, so plan on either having
> or also purchasing a nice computer (and with a compatible video card, check
> with the vendor as far as which cards work best).
>
> Most of these tools are under development, so who knows what will be the
> best next year!
>
> On Wed, Nov 13, 2019 at 7:32 AM Andreas Bruckbauer <
> [hidden email]> wrote:
>
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> >
> > Dear all,   I am interested to hear your opinions regarding image
> analysis
> > software for processing of larger datasets 3D and 4D datasets. How
> > do Imaris, Arivis and Aivia (maybe others?) compare? I am aware of Icy
> and
> > Fiji, but often find that we are hitting the limits when working with
> large
> > files e.g. 20 GB - 50 GB, but not the TB range yet. On the other hand we
> > often have to go back to Fiji to do some more complex analysis. This
> would
> > be for a light microscopy facility setting.    Best wishes   Andreas
> >
>


--
Best,

Gary Laevsky, Ph.D.
Director, Confocal Imaging Facility
Nikon Center of Excellence
Co-Founder, North Atlantic Microscopy Society (NAMS)
https://namsmicroscopy.com/
Dept. of Molecular Biology
Washington Rd.
Princeton University
Princeton, New Jersey, 08544-1014
(O) 609 258 5432
(C) 508 507 1310

North Atlantic Microscopy Society Annual Meeting at Princeton University,
Nov. 1, 2019.