Posted by
Zdenek Svindrych-2 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/2P-laser-coupled-to-a-DMD-tp7590172p7590174.html
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Hi Gary,
it's certainly not easy :-). If you're thinking about turning just few
pixels on that would define the locations of photoactivation (so the DMD is
a mask that is conjugate to the image plane), then, no, this won't work. At
least not over any reasonable field of view. The throughput would be too
low and you won't get enough peak power for two-photon absorption to
manifest.
If you're thinking to encode a diffraction pattern on the DMD that would
steer your beam (single spot in the image plane at a time), this would
probably work, but I don't know any commercial product like this, as
galvo-based steering is more efficient, simpler, cheaper; only quite a bit
slower. There are vis-laser solutions available commercially, but those use
LCOS for better efficiency.
From the power-handling point of view, you won't really damage a DMD or
LCOS SLM with a 1 W average power spread over the full chip. The peak power
won't matter as it's not focused to a spot on the DMD; the 80-or-so MHz
pulses just might disturb the electronics behind the pixels in some very
unfavorable cases.
Best, zdenek
Zdenek Svindrych, Dartmouth College
On Tue, Nov 19, 2019 at 3:34 PM Gary Laevsky <
[hidden email]>
wrote:
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>
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> *****
>
> Hi All,
>
> I THINK this can be done? DMD that can handle high power and appropriate
> reflective characteristics for the wavelength. Or could I do this with an
> SLM?
>
> We're looking to do non-linear optogenetic switching.
>
> If this is simple, please be gentle. I'm a biologist ...:)
>
> If it is simple, can you please direct me to the appropriate vendor.
>
> Thanks in advance.
>
> --
> Best,
>
> Gary Laevsky, Ph.D.
> Director, Confocal Imaging Facility
> Nikon Center of Excellence
> Co-Founder, North Atlantic Microscopy Society (NAMS)
>
https://namsmicroscopy.com/> Dept. of Molecular Biology
> Washington Rd.
> Princeton University
> Princeton, New Jersey, 08544-1014
> (O) 609 258 5432
> (C) 508 507 1310
>
> North Atlantic Microscopy Society Spring Meeting at UPENN, April 23, 2020.
>
--
--
Zdenek Svindrych, Ph.D.
Research Associate - Imaging Specialist
Department of Biochemistry and Cell Biology
Geisel School of Medicine at Dartmouth