http://confocal-microscopy-list.275.s1.nabble.com/2P-laser-coupled-to-a-DMD-tp7590172p7590175.html
correlation. For spatial (as in holographic), as others have said, you
temporal encoding to move the probe in the axial domain. DMDs are actually
> *****
> To join, leave or search the confocal microscopy listserv, go to:
>
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy> Post images on
http://www.imgur.com and include the link in your posting.
> *****
>
> Hi Gary,
>
> it's certainly not easy :-). If you're thinking about turning just few
> pixels on that would define the locations of photoactivation (so the DMD is
> a mask that is conjugate to the image plane), then, no, this won't work. At
> least not over any reasonable field of view. The throughput would be too
> low and you won't get enough peak power for two-photon absorption to
> manifest.
>
> If you're thinking to encode a diffraction pattern on the DMD that would
> steer your beam (single spot in the image plane at a time), this would
> probably work, but I don't know any commercial product like this, as
> galvo-based steering is more efficient, simpler, cheaper; only quite a bit
> slower. There are vis-laser solutions available commercially, but those use
> LCOS for better efficiency.
>
> From the power-handling point of view, you won't really damage a DMD or
> LCOS SLM with a 1 W average power spread over the full chip. The peak power
> won't matter as it's not focused to a spot on the DMD; the 80-or-so MHz
> pulses just might disturb the electronics behind the pixels in some very
> unfavorable cases.
>
> Best, zdenek
>
> Zdenek Svindrych, Dartmouth College
>
>
> On Tue, Nov 19, 2019 at 3:34 PM Gary Laevsky <
[hidden email]>
> wrote:
>
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> >
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy> > Post images on
http://www.imgur.com and include the link in your
> posting.
> > *****
> >
> > Hi All,
> >
> > I THINK this can be done? DMD that can handle high power and appropriate
> > reflective characteristics for the wavelength. Or could I do this with
> an
> > SLM?
> >
> > We're looking to do non-linear optogenetic switching.
> >
> > If this is simple, please be gentle. I'm a biologist ...:)
> >
> > If it is simple, can you please direct me to the appropriate vendor.
> >
> > Thanks in advance.
> >
> > --
> > Best,
> >
> > Gary Laevsky, Ph.D.
> > Director, Confocal Imaging Facility
> > Nikon Center of Excellence
> > Co-Founder, North Atlantic Microscopy Society (NAMS)
> >
https://namsmicroscopy.com/> > Dept. of Molecular Biology
> > Washington Rd.
> > Princeton University
> > Princeton, New Jersey, 08544-1014
> > (O) 609 258 5432
> > (C) 508 507 1310
> >
> > North Atlantic Microscopy Society Spring Meeting at UPENN, April 23,
> 2020.
> >
>
>
> --
> --
> Zdenek Svindrych, Ph.D.
> Research Associate - Imaging Specialist
> Department of Biochemistry and Cell Biology
> Geisel School of Medicine at Dartmouth
>
Benjamin E. Smith, Ph. D.