Re: 2P laser coupled to a DMD?

Posted by Christian Wilms on
URL: http://confocal-microscopy-list.275.s1.nabble.com/2P-laser-coupled-to-a-DMD-tp7590172p7590179.html

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Hi Gary,

As others have already said, a DMD won't easily get you the power levels that you require for 2P excitation of most optogenetic tools. LCoS SLMs are the tool of choice for this. Many commercial suppliers of multiphoton microscopes have licensed the technology and now sell such add-ons (or have them in development), so it might be worth asking the manufacturer of your system.

If you are more the DIY type, have a look at the work by Volodymyr Nikolenko and Darcy Peterka from Rafael Yuste's lab at Columbia. This paper might be a good introduction: https://doi.org/10.3389/neuro.04.005.2008

Darcy and his colleagues (among many others) are really helping push this technology and are just a couple of hours from you and are the holders of the main patent covering this technology.

In addition to the actual SLM, if you want to target more than a few spots simultaneously, you should also be looking into suitable lasers, as the ones commonly used in multiphoton imaging tend not to hit the required parameters for most opsins.

Best, Christian

Dr. Christian Wilms / Research & Development Manager
[hidden email] / [phone]
www.scientifica.uk.com

Take a look at our NeuroWire blog to see our latest news, guides, videos and more.

> -----Original Message-----
> From: Gary Laevsky <[hidden email]>
> Sent: 19 November 2019 20:33
> Subject: 2P laser coupled to a DMD?
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi All,
>
> I THINK this can be done?  DMD that can handle high power and appropriate
> reflective characteristics for the wavelength.  Or could I do this with an SLM?
>
> We're looking to do non-linear optogenetic switching.
>
> If this is simple, please be gentle.  I'm a biologist ...:)
>
> If it is simple, can you please direct me to the appropriate vendor.
>
> Thanks in advance.
>
> --
> Best,
>
> Gary Laevsky, Ph.D.
> Director, Confocal Imaging Facility
> Nikon Center of Excellence
> Co-Founder, North Atlantic Microscopy Society (NAMS)
> https://namsmicroscopy.com/ Dept. of Molecular Biology Washington Rd.
> Princeton University
> Princeton, New Jersey, 08544-1014
> (O) 609 258 5432
> (C) 508 507 1310
>
> North Atlantic Microscopy Society Spring Meeting at UPENN, April 23, 2020.