http://confocal-microscopy-list.275.s1.nabble.com/2P-laser-coupled-to-a-DMD-tp7590172p7590181.html
> Thank you all!
>
> From what I understand, this is totally doable. I'll need to use an SLM
> as opposed to a DMD.
>
> Clarification and additional questions;
>
> This is for spatial non-linearity. I want to light up "a" cell in a
> biofilm/embryo/tissue, at "depth," less than 100 um. I'm not going to be
> able to do this with 1P, right? So this WILL limit the selection of
> opsins. If I get something like an InSight, 1300 should get me to the
> red-shifted? I just emailed Darcy:)
>
> I'm surprised about the power requirement. I would have thought low power
> would be sufficient?
>
> I'm thinking I have two options; put the SLM between a scan head and the
> scope, with divergent then collimating optics to the SLM, and park the beam
> of the scan head on the SLM? Or, skip the scan head and couple the
> laser/SLM directly to stand. The former will give me additional
> capability, but I'm not sure if that light path is viable?
>
> Thank you ALL very much!
>
>
>
> On Wed, Nov 20, 2019 at 4:57 AM Christian Wilms <
>
[hidden email]> wrote:
>
>> Hi Gary,
>>
>> As others have already said, a DMD won't easily get you the power levels
>> that you require for 2P excitation of most optogenetic tools. LCoS SLMs are
>> the tool of choice for this. Many commercial suppliers of multiphoton
>> microscopes have licensed the technology and now sell such add-ons (or have
>> them in development), so it might be worth asking the manufacturer of your
>> system.
>>
>> If you are more the DIY type, have a look at the work by Volodymyr
>> Nikolenko and Darcy Peterka from Rafael Yuste's lab at Columbia. This paper
>> might be a good introduction:
https://doi.org/10.3389/neuro.04.005.2008>>
>> Darcy and his colleagues (among many others) are really helping push this
>> technology and are just a couple of hours from you and are the holders of
>> the main patent covering this technology.
>>
>> In addition to the actual SLM, if you want to target more than a few
>> spots simultaneously, you should also be looking into suitable lasers, as
>> the ones commonly used in multiphoton imaging tend not to hit the required
>> parameters for most opsins.
>>
>> Best, Christian
>>
>> Dr. Christian Wilms / Research & Development Manager
>>
[hidden email] / +44 (0)1825 749933
>> www.scientifica.uk.com
>>
>> Take a look at our NeuroWire blog to see our latest news, guides, videos
>> and more.
>>
>> > -----Original Message-----
>> > From: Gary Laevsky <
[hidden email]>
>> > Sent: 19 November 2019 20:33
>> > Subject: 2P laser coupled to a DMD?
>> >
>> > *****
>> > To join, leave or search the confocal microscopy listserv, go to:
>> >
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy>> > Post images on
http://www.imgur.com and include the link in your
>> posting.
>> > *****
>> >
>> > Hi All,
>> >
>> > I THINK this can be done? DMD that can handle high power and
>> appropriate
>> > reflective characteristics for the wavelength. Or could I do this with
>> an SLM?
>> >
>> > We're looking to do non-linear optogenetic switching.
>> >
>> > If this is simple, please be gentle. I'm a biologist ...:)
>> >
>> > If it is simple, can you please direct me to the appropriate vendor.
>> >
>> > Thanks in advance.
>> >
>> > --
>> > Best,
>> >
>> > Gary Laevsky, Ph.D.
>> > Director, Confocal Imaging Facility
>> > Nikon Center of Excellence
>> > Co-Founder, North Atlantic Microscopy Society (NAMS)
>> >
https://namsmicroscopy.com/ Dept. of Molecular Biology Washington Rd.
>> > Princeton University
>> > Princeton, New Jersey, 08544-1014
>> > (O) 609 258 5432
>> > (C) 508 507 1310
>> >
>> > North Atlantic Microscopy Society Spring Meeting at UPENN, April 23,
>> 2020.
>>
>
>
> --
> Best,
>
> Gary Laevsky, Ph.D.
> Director, Confocal Imaging Facility
> Nikon Center of Excellence
> Co-Founder, North Atlantic Microscopy Society (NAMS)
>
https://namsmicroscopy.com/> Dept. of Molecular Biology
> Washington Rd.
> Princeton University
> Princeton, New Jersey, 08544-1014
> (O) 609 258 5432
> (C) 508 507 1310
>
> North Atlantic Microscopy Society Spring Meeting at UPENN, April 23, 2020.
>
Dept. of Molecular Biology
North Atlantic Microscopy Society Spring Meeting at UPENN, April 23, 2020.