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Many thanks for all the replies and good comments, I now got test licenses, a powerful computer and am happily testing. It turns out that professional software can be much faster (~20x) than Fiji for the same task, due to efficient multitasking? But actually measuring the speed is not so straightforward as small changes to the parameters can make a big difference in speed. Sometimes the whole analysis comes to a stop and one does not know where it is hanging... And pre-fabricated pipelines can be very inflexible. best wishes Andreas -----Original Message-----
From: Steffen Dietzel <
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To: CONFOCALMICROSCOPY <
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Sent: Fri, 15 Nov 2019 9:37
Subject: Re: Software for 3D and 4D analysis
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Am 13.11.2019 um 23:33 schrieb Benjamin Smith:
> One other software package to consider is Avizo. When we bought it, it was
> one of the cheapest options, as well as one of the most powerful,
> especially for large data. Bonus points for the fact it lets you load and
> display multiple datasets simultaneously,
I don't have much to add to what has been said, except maybe this: Yes,
one instance of Imaris will open only one data set. But you can open
several instances of Imaris in parallel. Whether this is a fun thing to
do or not will depend on the power of the hardware.
Best
Steffen
--
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Steffen Dietzel, PD Dr. rer. nat
Ludwig-Maximilians-Universität München
Biomedical Center (BMC)
Head of the Core Facility Bioimaging
Großhaderner Straße 9
D-82152 Planegg-Martinsried
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http://www.bioimaging.bmc.med.uni-muenchen.de