http://confocal-microscopy-list.275.s1.nabble.com/Spinning-disk-or-new-point-scanning-confocals-for-live-imaging-of-3D-engineered-tissues-tp7590297p7590310.html
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> Hi,
> I will start my ERC-funded lab in Italy in 2020, which means I am fortunate
> to be shopping for a confocal instrument right about now.
>
> Since I do mostly live experiments (more details below) I was going to get
> a spinning disk system. But, I realized that the new scanning confocals are
> also relatively fast and gentle. The question is how much (if at all)
> slower and harsher are they with respect to spinning disks?
>
> More details:
> - I do a lot of live experiments (traction force microscopy and
> voltage/calcium-sensitive dyes) on engineered cells (300x300 um FOV) and
> tissues (600x600 um FOV) in 2D or 3D (<300 um thick) that I complement with
> immunostainings after fixation. Needed acquisition rates go from <1 fps to
> 100s depending on the application.
>
> - Originally, I was oriented towards a spinning disk confocal
> (Yokogawa/SORA, Crest) and was looking at ways to deal with the issues of
> confocality on the 3D tissue (600x600x300 um volumes) case. But, I realized
> all vendors have new point scanning confocal (980+Airyscan2, FV3000, A1R)
> with resonant scanners that acquire full frames/ROIs at 10s/100s of fps
>
> - Of course, structured illumination (Elyra-7, N-SIM) and light-sheet
> microscopes (QuVi) are also appealing but relatively untested in my
> applications of interests....
>
> The application specialists from all vendors in my area have been great to
> work with but since my lab is not up and running, yet, I can't demo these
> systems directly. Therefore, I could use help and feedback, especially from
> people who have had related experiences in the recent past.
>
> Thanks,
> Francesco
>
> --
> Francesco S. Pasqualini
> Visiting Professor University of Pavia
> Associate Harvard University
>
> tel: +39 351-521-7788 (IT)
> tel: +1 617-401-5243 (USA)
>