Re: Spinning disk or (new) point scanning confocals for live imaging of 3D engineered tissues?

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Dear Francesco,

for applications you describe a spinning disk system would very likely be the only choice: as Sylvie mentioned with a point scanning system you would end up making compromises.

In the past two years we have installed multiple laser-free structured illumination spinning disk systems from Aurox (Clarity: not to mix up with the tissue clearing method). The application range is pretty wide and runs from localization-based super resolution (SRRF) to fast imaging (at least 100 Hz is possible) to whole organism imaging.

As the system is camera-based and collects both in-focus and out of focus light it is extremely photon efficient, it allows the laser-free operation which is of course gentle to your samples.

Since Clarity is an economic add-on module that can be fitted to almost any microscope frame it has a very high investment value. Certainly worth having a look:

www.aurox.co.uk

Best regards,

Mika