http://confocal-microscopy-list.275.s1.nabble.com/COVID19-Instrument-cleaning-protocols-tp7590586p7590618.html
Thanks Gustav for sharing and everyone for the good contributions, great to have this important discussion.
I don't understand the no-gloves rule in the times of Covid-19, it assumes that the gloves are contaminated and the hands are clean. But now we are facing the situation that the microscope, keyboard, mouse can be dirty and people want to keep their hands clean. I think it should be gloves only policy. When using no-gloves would rely on everyone following the rules perfectly, one infected person coughing into their hand and touching the mouse or focus knob could spread the disease. Just my thoughts.
Neil Anthony, our core director at Integrated Cellular Imaging (ICI) facility at Emory University has prepared a template with graphics for instrument cleaning. This can be customized for your core/institution.
It encompasses suggestions put forward by many of you in this discussion thread
> On Mar 15, 2020, at 4:14 PM, Jacqueline Ross <
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> Hi everyone,
>
> This has been interesting reading. We currently have 8 confirmed and 3 probable cases in New Zealand. I know this is a very small number compared with many other countries and our sympathies are with you all. However, winter is coming and our government has now stepped up with stronger restrictions. Our university has banned all international travel and recalled all staff who are currently overseas.
>
> In regard to our core facility, I intend to send an email to all microscope users reminding them that they must wash their hands thoroughly with soap (preferred option) or use hand sanitizer before leaving the lab. This is our normal practice.
>
> However I will also be asking all users to also wash their hands before using the microscopes, i.e. after entering the main lab. Based on these discussions, I intend to also ask people to clean the eyepieces before and after using the microscope.
>
> I am also considering removing all plastic/rubber eye cups from the eyepieces as an extra precaution. What do people think about that idea?
>
> Like Michael, we have a "no gloves rule" apart from when people are working with live samples. They can wear gloves when handling their specimen but only when putting it onto and off the microscope
> Gloves must be removed before touching any other components such as focus wheel, joy stick, etc.
>
> For us, it's not practicable for us to clean microscopes between users and I wouldn't feel comfortable leaving anything like bleach around in case some of the more anxious users decided to spray it around.
>
> We use Accel wipes (peroxide) for benches so I will probably wipe those myself each day and also all door handles. However if everyone observes good hand hygiene, the internal room handles should be low risk.
>
> Kind regards,
>
> Jacqui
>
> Jacqueline Ross
> Lead Technologist
> Optical Microscopy & Image Analysis
> Biomedical Imaging Research Unit (BIRU)
> School of Medical Sciences
> Faculty of Medical & Health Sciences
> The University of Auckland
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> Telephone: Ext 87438; DDI: +64 9 923 7438
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> ________________________________________
> From: Confocal Microscopy List [
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> Sent: 15 March 2020 08:26
> To:
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> Subject: Re: COVID19: Instrument cleaning protocols
>
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> I have read many, but not all of, the messages in this thread, so apologies if some redundancy.
>
> The following response is a few tips for microscope decontamination for BSL-2 (and in some cases BSL-3), not specifically for the corona virus, but I believe these points apply and some of them are for equipment protection while also fulfilling the goal of microorganism or virus decontamination. They could be extended to decontamination of scopes and ancillary equipment as per users who may be carrying contagious illnesses. I am writing from the point of view of a core facility with scopes used by many people and in scattered locations with minimal or no supervision. These are more practical tips we have learned (the hard way) for operating as part of more thorough protocols.
>
> Paper goods: We do not allow commercial tissues in the lab. We have had people with colds come to use the microscopes and bring their own box of tissues with them to wipe their noses while using our equipment. (Yes, this is true.) They will use these tissues on the equipment if they are within reach. Tissues are likely to scratch optics and leave dust everywhere. Paper goods need to be limited to lens tissue and wood fiber only Kimwipes.
>
> 70% EtOH and 10% bleach must be kept in bottles that look completely different. People will get them confused otherwise and bleach sprayed on optics is not good. A bottle with a white top and a bottle with a red top with Sharpie marker writing on the side are not different enough. We solve this problem by supplying the 70% EtOH in clear hand labeled bottles and the bleach in prepackaged Clorox bottles. (The clear bottles are marked with Sharpie and then clear packing tape over the writing to prevent the ink from being washed off because, like immersion oil somehow getting applied to every surface, EtOH will run down the side of the bottle. The Clorox bottles are also labeled in BIG letters, Microscopy, to reduce "borrowing" of them; when you need the bleach, you need it next to the scope, not in somebody else's lab.)
>
> Using prepackaged bleach has another benefit. It is buffered so it remains active longer. The downside of this is that it may leave a fine power residue. However, this is preferential to having people mistaking bottles and spraying bleach on optics. The surfaces may be wiped down a second time with EtOH, water, or lens cleaner to remove the powder.
>
> 70% EtOH may damage plastics (other people have mentioned this). Beware overuse on Plexiglas boxes, plastic knobs, and painted surfaces. We have had environmental chambers on scopes develop networks of microcracks due to too much EtOH. If you want to decontaminate these surfaces, we recommend 10% bleach.
>
> Other people have mentioned methanol and its toxicity. It is not used as an antiseptic. Staff use this very sparingly for cleaning optics. There is no methanol available where general users may access it.
>
> People need to be told repeatedly not to spray directly onto the microscope. Demonstration training is necessary for people to see how to saturate a Kimwipe and how to wipe once and dispose. Perhaps the single most misunderstood aspect of cleaning is that the first wipe removes and the second wipe or circular motion smears it around. I try to explain this with analogies such as proper wiping when changing an infant's diaper, the pink stuff in The Cat In The Hat Comes Back, or fine art painting wet-in-wet, but these references are often not shared by our clients. (This is similar to the training how to clean oil off immersion lenses.)
>
> Use of gloves is another big problem, but here are some highlights. Environmental Health and Safety has clear rules that the microscopes may not be used with gloves on. One argument is that prohibiting gloves makes people more cognizant of safe handling. Regardless, this blanket prohibition removes our having to argue with people over glove use. The main point is that if the samples are too dangerous to use with gloves off, then they can't be used on the microscope. However, cleaning up spills and handling cleaning solvents may require use of gloves. The final cleaning should be such that it is safe for anyone to touch without gloves. People really do need to be reminded that other people will be using the resources after them and there is a responsibility to make it safe for the next users. We supply gloves in the room. We keep the rooms stocked with gloves from small to X-large. People will reuse the same gloves if you don't give them a surplus of gloves. People need to be explicitly taught to peel off gloves inside-out and toss in trash or they will neatly pull off each finger and lay the contaminated glove on a table for reuse.
>
> Any instrument should be clean for immediate subsequent use by someone else.
> However, time is also decontamination for many pathogens.
>
> Whereas these points are part of routine BSL-2 protocols, they could be extended to protocol(s) for wiping down exterior of equipment, ancillary equipment including keyboards, mice, mouse pads, furniture such as tables, chairs, doorknobs, the spot on the door everyone pushes on (look at an angle to find the worn or dirty spot), etc.
>
> If you or specific staff are going to do the decontamination, then most of the points above are unnecessary. If unsupervised users will be directed to clean up after each use, I recommend heeding the above. All training would need to be demonstration. Ultimately, if you want decontamination done strictly your way, you need to do it yourself.
>
> (BTW, this email was written in New Rochelle, NY, two miles from where Thomas Paine wrote Common Sense.)
>
> Cheers-
>
>
>
> Michael Cammer, Sr Research Scientist, DART Microscopy Laboratory
>
> NYU Langone Health, 540 First Avenue, SK2 Microscopy Suite, New York, NY 10016
>
>
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> ________________________________
> From: Confocal Microscopy List <
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> Sent: Thursday, March 12, 2020 5:08 PM
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> Subject: Re: COVID19: Instrument cleaning protocols
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> Perhaps temporarily removing the rubber eye cups from the eyepieces would better facilitate cleaning between users for now?Sent from my Verizon, Samsung Galaxy smartphone
> -------- Original message --------From: Louis Kerr <
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[hidden email]>> Date: 3/12/20 12:30 PM (GMT-08:00) To:
[hidden email] <mailto:
[hidden email]> Subject: Re: COVID19: Instrument cleaning protocols *****To join, leave or search the confocal microscopy listserv, go to:
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https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=MlK8WMG06xfxzAmZVROsxqzZnioy-Jx4N6o1Ee17q2M&s=jYa_lp2KyvbjFXUs4LHG4Ai53otwmDG3p01p0lIsFvU&e=> and include the link in your posting.*****I need to add a correction to my earlier statement about the concentration of hydrogen peroxide. The stock is 8% but is diluted to either 0.25 or 0.375%. We also use a health care variety for other uses that is 0.5%.Thanks again,LouieLouie Kerr | Director, Imaging Services; Staff ScientistMarine Biological Laboratory, 7 MBL Street, Woods Hole, MA 02543508-289-7273 |
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[hidden email] <mailto:
[hidden email]>>Sent: Wednesday, March 11, 2020 2:19 PMTo: Glen MacDonald <
[hidden email] <mailto:
[hidden email]>>;
[hidden email] <mailto:
[hidden email]><
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[hidden email]>>Subject: Re: COVID19: Instrument cleaning protocolsI agree that >60% Ethanol degrades the finish and plastics/rubber over time. We have routinely used Ecolabs Peroxide Multi Surface Cleaner and Disinfectant. It contains 8% Hydrogen Peroxide.Very useful discussion as I am currently writing up internal recommendations as well.LouieLouie Kerr | Director, Imaging Services; Staff ScientistMarine Biological Laboratory, 7 MBL Street, Woods Hole, MA 02543508-289-7273 |
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[hidden email]>> on behalf of Glen MacDonald <
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[hidden email]>>Sent: Wednesday, March 11, 2020 1:53 PMTo:
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[hidden email]>>Subject: Re: COVID19: Instrument cleaning protocols*****To join, leave or search the confocal microscopy listserv, go to:
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https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=MlK8WMG06xfxzAmZVROsxqzZnioy-Jx4N6o1Ee17q2M&s=jYa_lp2KyvbjFXUs4LHG4Ai53otwmDG3p01p0lIsFvU&e= <
https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=MlK8WMG06xfxzAmZVROsxqzZnioy-Jx4N6o1Ee17q2M&s=jYa_lp2KyvbjFXUs4LHG4Ai53otwmDG3p01p0lIsFvU&e=> and include the link in your posting.>> *****>>>> Dario/Cesare-->>>> THANK YOU BOTH very much for emphasizing the importance of decontaminating>> the eye-pieces. It's obvious in retrospect but I was focussed on the>> computer keyboard, mouse, etc. --Are you using 80% ethanol on the>> eye-pieces?>>>> Best wishes to you and all our other colleagues. Good luck. Please keep>> us posted on developments or recommendations.>>>> And thank you to all those working to control this beast.>>>> Martin Wessendorf>>>> On Wed, Mar 11, 2020 at 8:47 AM Cesare|ALEMBIC <
[hidden email] <mailto:
[hidden email]>> wrote:>>>>> *****>>> To join, leave or search the confocal microscopy listserv, go to:>>>
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https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.umn.edu_cgi-2Dbin_wa-3FA0-3Dconfocalmicroscopy&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=MlK8WMG06xfxzAmZVROsxqzZnioy-Jx4N6o1Ee17q2M&s=QXvW_DdKX3U_SetwYRZeK3plPj6S8qo29KTtEAf-530&e=> >>> Post images on
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https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=MlK8WMG06xfxzAmZVROsxqzZnioy-Jx4N6o1Ee17q2M&s=jYa_lp2KyvbjFXUs4LHG4Ai53otwmDG3p01p0lIsFvU&e=> and include the link in your posting.>>> *****>>>>>> Dear Javier,>>>>>> here in Milano, as already mentioned by Mario and Dario, we are still>>> available (althouhg at lower level as well) but we implemented, according>>> to Institute Direction, different measures to reduce as much as possibile>>> the access to the Facility and we recommended to our Users to perform only>>> urgent and undelayable experiments.>>>>>> Facility Staff:>>> we reduced staff members daily present to the minimum is possible>>> Facility entrance:>>> - Instruments will be available only from 08:00 to 18:00 from Monday to>>> Friday (instead 24/24h, 7/7d) and instrument usage is not allowed on>>> holidays and outside of this time schedule.>>> - A disposable glove dispenser has been set up outside the entrance door>>> of the facility. All Users must wear them before entering the Facility and>>> leave them in a dedicated container as they finish their activities.>>> - the entrance to the Facility will be regulated in order to avoid any>>> form of crowding that sees multiple subjects in the same room at a distance>>> of less than 1.8 meters.>>> - Instruments use, including microscopes mouse and keyboards, must be done>>> by wearing gloves and, as much as possible, with the own protective glasses>>> (please, remember that Covid-19 seems particularly transmitted by aerosol>>> contact with conjunctive).>>> - At the end of each session, each User must notify it to the Facility>>> Staff who will disinfect the exposed parts with Ethanol 70% (microscope,>>> mouse, keyboards, touchscreens etc. all parts that could be usually touched>>> by hands or eyes, using a disposable cloths.>>> - we require only one person per Instrument and, for the same reason,>>> trainings and assisted uses are temporarily suspended.>>>>>>>>> Instruments maintenance :>>> no different procedures respect usual maintenance cleaning>>>>>> I hope this can be useful and a special hello to my "Milanesi" collegues!>>>>>> Cesare>>>>>>>>> Dr. Cesare Covino, M.Sc.>>> Facility Manager>>> ALEMBIC>>> Advanced Light and Electron Microscopy BioImaging Center>>> San Raffaele Scientific Institute>>> DIBIT 1 S2B4a>>> via Olgettina 58, 20132 - Milano - Italy>>> Tel +39-022643-4636(others -4640, -4641)>>> Fax +39-022643-4646>>> e-mail:
[hidden email] <mailto:
[hidden email]><mailto:
[hidden email] <mailto:
[hidden email]>>>>> ALEMBIC webpage:
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https://urldefense.proofpoint.com/v2/url?u=http-3A__alembic.hsr.it&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=MlK8WMG06xfxzAmZVROsxqzZnioy-Jx4N6o1Ee17q2M&s=m5wSxuxSuHqOy3Vl8RsEh05s0YxuC2hgPgLDCQqowZg&e=> >>>>>>>>> Il 11/03/2020 10:39, F Javier Diez Guerra ha scritto:>>> *****>>> To join, leave or search the confocal microscopy listserv, go to:>>>
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https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.umn.edu_cgi-2Dbin_wa-3FA0-3Dconfocalmicroscopy&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=MlK8WMG06xfxzAmZVROsxqzZnioy-Jx4N6o1Ee17q2M&s=QXvW_DdKX3U_SetwYRZeK3plPj6S8qo29KTtEAf-530&e=> >>> Post images on
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https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=DwIGaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=hUBj2D5n6oKThx2L01qn8IORZb5f-ruLVXPmQ1zQNnM&m=MlK8WMG06xfxzAmZVROsxqzZnioy-Jx4N6o1Ee17q2M&s=jYa_lp2KyvbjFXUs4LHG4Ai53otwmDG3p01p0lIsFvU&e=> and include the link in your posting.>>> *****>>>>>> Dear listers,>>>>>> In Madrid, teaching and many other collective activities have been>>> suspended for 15 days because of COVID19. On the other hand, research>>> institutes continue with their normal activity although at a lower level.>>>>>> In order to reduce as much as possible the spread of the virus, our>>> advanced bioimaging facility is considering to implement additional>>> measures or protocols (beyond common sense).>>>>>> We would be very grateful if you could share your advice, suggestions and>>> protocols especially regarding the cleaning procedures of the instruments>>> (binocular, focus knobs, switches, stage joysticks, etc) and other measures>>> that affect to the users (wear gloves, etc). We are interested to know>>> about easy and effective cleaning procedures that would not be harmful for>>> the optics, electronics, etc.>>>>>> Thanks for your input>>>>>> Best regards>>>>>>>>>>>>>>>>>> Rispetta l'ambiente: non stampare questa mail se non ? necessario.>>> Respect the environment: print this email only if necessary.>>>>> .