Posted by
Michelle Peckham on
URL: http://confocal-microscopy-list.275.s1.nabble.com/New-practical-tutorial-on-quantitative-confocal-microscopy-now-out-in-Nature-Methods-tp7590685p7590692.html
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Great paper!
Should be required reading.
I like the concluding remarks too...
All best
Michelle
On 31/03/2020, 21:41, "Confocal Microscopy List on behalf of Alison J. North" <
[hidden email] on behalf of
[hidden email]> wrote:
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Dear fellow microscopists,
We are delighted to announce the publication of a new “Tutorial: guidance for quantitative confocal microscopy” in Nature Protocols, compiled by James Jonkman, Claire Brown, Graham Wright, Kurt Anderson and Alison North. This paper, which somehow gradually transformed from a small practical guide into quite an epic, was spearheaded by James Jonkman and ended up as a collaboration by facility heads across three continents. We hope that you will find it a useful addition to the reading material you hand out to your confocal microscopy trainees – obviously it cannot compare to our still-beloved Pawley confocal handbook but it is a lot quicker to read! There will undoubtedly be parts that some of you won’t agree with – truth be told, we often disagreed amongst ourselves, ha ha, but we trust that these animated discussions led to a fairer and more representative consensus in the end! We hope that this will provide some light reading for your facility users and other imaging scientists while they are tied to home, as well as a good introduction for future new microscopists. It’s worth noting that much of the paper is not only useful for confocal microscopy – the sections on specimen preparation, choosing the right microscope, statistics, and pilot projects, among others, should also appeal to those foraying into other microscopy techniques. In addition, we listed almost 100 references pointing people to more in depth reading on many of the topics.
You can download a PDF via the following link, if you have institutional access to Nature Protocols:
https://www.nature.com/articles/s41596-020-0313-9
If you don’t have full access, you can still view a full copy via this SharedIt link (
https://rdcu.be/b3ko0<
https://urldefense.proofpoint.com/v2/url?u=https-3A__rdcu.be_b3ko0&d=DwMGaQ&c=JeTkUgVztGMmhKYjxsy2rfoWYibK1YmxXez1G3oNStg&r=RBx0-WJrAO5vwSOLNmFbqYvikvIZS5ns3-USwvMOuLo&m=r6iuWA3giW4k5tQGtW0zUn4RoMDOmsT3tilY2MRvuy4&s=uaLFjZqwbnNztkoEWPrUmw4unw8TBfsLT-KYBavPL0w&e=>) and/or contact the authors for a copy.
Happy reading and imaging, and stay safe at home, wherever you are!
James, Claire, Graham, Kurt and Alison