Re: Intensity Calibration Microspheres

Posted by Claire Brown on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Intensity-Calibration-Microspheres-tp7590776p7590791.html

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The imaging was done on over 40 different microscopes with different magnifications, immersion medium and NA and instrument seeings.
This is why we used the intensity ratio. the dim and bright beads were in the same sample though.