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Re: Intensity Calibration Microspheres

Posted by Claire Brown on Apr 20, 2020; 11:02pm
URL: http://confocal-microscopy-list.275.s1.nabble.com/Intensity-Calibration-Microspheres-tp7590776p7590792.html

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They are the same size, but perhaps the brighter ones could heat up more? Not sure how much heating you might get with a green laser though.

Could also be flow get the max intensity at the middle of the sphere but in microscopy we measure the whole thing. however, I would think that any difference like that would disappear with the ratio.