Size of a bleach point

Posted by xavier Sanjuan on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Size-of-a-bleach-point-tp7590808.html

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Dear listers,

When using the Bleach Point feature in the Leica confocal systems (I'm sure
it also exists in the other brands), I don't know how to measure the
surface of the bleached area. I figure out the wavelength of the laser beam
and the NA of the lens affect the size of that area, so would it be correct
to use the resolution formula to calculate it?  Or am I mixing up things
here?

Thank you very much,

Xavi.

___________________________________

*Xavier Sanjuan*
Advanced Light Microscopy Unit

Parc de Recerca Biomèdica de Barcelona
Doctor Aiguader, 88
08003 Barcelona - Spain
Tel: + 34 93 316 0195 (ext 1195 dins el PRBB)
Fax: + 34 93 316 09 01
E-mail: [hidden email]
Web:
http://www.crg.eu/en/core/programmes-groups/advanced-light-microscopy-unit